HIF-2α在妊娠期肝内胆汁淤积症患者胎盘中的表达及意义

Expression and significance of hypoxia-inducible factor-2α in placenta in intrahepatic cholestasis of pregnancy

目的：检测妊娠期肝内胆汁淤积症（ICP）患者胎盘组织缺氧诱导因子HIF-2α蛋白的含量,探讨其在ICP胎儿宫内缺氧中的作用。方法：选取2010年2-8月在弋矶山医院剖宫产分娩的40例孕妇为研究对象,其中正常足月孕妇10例,ICP轻度组15例,重度组15例。采用蛋白免疫印迹法检测各组孕妇胎盘组织中HIF-2α蛋白的表达水平。结果：3组孕妇HIF-2α蛋白表达差异有统计学意义（P〈0.05）,轻、重度ICP组HIF-2α蛋白表达较正常对照组有统计学意义（P〈0.05）;轻、重度ICP组间HIF-2α蛋白表达差异无统计学意义（P〉0.05）。结论：ICP患者HIF-2α蛋白在胎盘组织中的高表达,可能在胎盘-胎儿系统对缺氧的自稳和代偿反应中起重要的调节作用。

Objective： To investigate the role of hypoxia-inducible factor-2α（ HIF-2α） protein in placenta in intrahepatic cholestasis of pregnancy（ ICP）.Methods： Forty women undergone cesarean section delivery were included between February and August of 2010 in our hospital. Of them,10 were normal full-term pregnancy women,15 were complicated with mild ICP and another 15,with severe ICP. Western blot was performed determine the protein level of placental HIF-2α. Results： HIF-2α level was different among the three groups（ P〈0. 05）,and higher in mild and severe ICP group as compared with normal controls（ P〈0. 05）,yet difference was not significant between mild and severe ICP group（ P〈0. 05）. Conclusion： HIF-2α protein is highly expressed in placenta in ICP pregnancy,suggesting that it play an important role in regulating the compensatory homeostatic response to hypoxemia of placenta-fetus system.