BolSDG8 RNA干扰载体构建和拟南芥的遗传转化

RNA Interference Vector Construction of Bol SDG8 and Genetic Transformation of Arabidopsis thaliana

拟南芥SDG8通过调控开花关键基因FLC位点上H3K36的甲基化水平促进其转录表达进而抑制植株早花。甘蓝Bol SDG8是拟南芥SDG8的同源基因,经比对,选择一段约为359 bp的保守序列,命名为Bol SDG8-RNAi,通过构建RNA干扰载体,并将其转化至野生型拟南芥(Col-0)中,获得了稳定遗传的转基因植株。通过对T3代转基因植物的表型观察,开花天数及莲座叶数目的统计分析,发现Bol SDG8 RNA干扰转基因植株表现出与拟南芥sdg8-2突变体相似的生物学表型,即植株弱小,明显早花,暗示Bol SDG8与拟南芥SDG8在调控植物开花上具有相似的生物学功能,为进一步深入研究甘蓝Bol SDG8的生物学功能奠定基础。

Arabidopsis SDG8 specifically regulates methylation of H3K36 on the flowering key gene FLC, to promote its transcriptional expres- sion and inhibit early flowering in plants. BolSDG8 of Brassica oleraeea is a homologous gene of SDG8 of Arabidopsis thaliana. By blasting, a conserved sequence of 359 bp named BolSDGS-RNAi was selected via construction of RNA interference vector and genetic transformation into wild-type Arabidopsis plants （Col-0）, and obtained one transgenic plant with stable inheritance. Through phenotype observation of transgenic plants in T3 , and analysis of days to bolting and rosette leaves number, it was found that BolSDG8 RNAi transgenie plants in Arabidopsis showed similar biological phenotypes to sdgS-2 mutants, weak and significantly early flowering, laying a foundation for further study of biological function of BolSDGS.