2种在组织切片上鉴别兔脑炎原虫的新方法

Two New Staining Methods to Identify Encephalitozoon cuniculi on Tissue Section

[目的]探索在病理组织切片上鉴别兔脑炎原虫的新方法。[方法]用改良的革兰氏染色法和甲基绿派诺宁染色法对病兔脑组织的病变进行染色与鉴别。[结果]普通染色法虽然可清晰观察到脑组织的病理变化,但却不易检出虫体。采用改良的革兰氏染色法,不仅病理结构比较明显,而且可清晰看到染成蓝色的脑炎原虫。用甲基绿派诺宁染色,位于上皮样细胞内的脑炎原虫被染成紫红色。[结论]改良的革兰氏染色法和甲基绿派诺宁染色法对病理组织中的兔脑炎原虫具有良好的染色作用,有助于对兔脑炎原虫病的快速确诊。

[ Objective ] The research aimed to discuss new methods of identify Encephalitozonoos curiculi on tissue sections. [ Method ] Using improved Gram staining method and methyl green pyronin staining method, the pathological sections of diseased rabbits were stained and identified. [ Result] The pathological changes of brain tissue on sections could be clearly observed, but parasites were not examined in pathological brain tissues stained by common staining method. When the pathological section was stained by improved Gram staining method, the pathological changes of brain tissue were not only stained very clearly, but blue parasites were also found in lesion brain tissues. The parasites located in epithelioid cells were stained as zinzolin ones by methyl green pyronin staining method. [ Conclusion ] The improved Gram staining method and methyl green pyronin staining method performed good staining effects of E. cuniculi in pathological sections, which were conductive to rapidly diagnose the en- cephalitozoonosis in rabbit.