HPLC同时测定鱼腥草芩蓝合剂中3种活性成分的含量

Simultaneous Determination of Three Ingredients in Yuxingcao Qinlan Mixtures by HPLC

目的建立HPLC测定鱼腥草芩蓝合剂中绿原酸、黄芩苷和黄芩素的含量。方法采用KromasiL^-100 C18色谱柱（4.6 mm×250 mm,5μm）,流动相为甲醇-0.2%磷酸梯度洗脱,流速为1.0 m L·min^-1,检测波长为322 nm,柱温为35℃。结果绿原酸、黄芩苷和黄芩素线性范围分别为0.022 4-1.12μg（R2=0.999 7）,0.045 8-2.29μg（R^2=0.999 5）和0.031 8-1.59μg（R2=0.999 9）;3种成分的平均回收率分别为99.45%（RSD=1.4%）,99.37%（RSD=2.0%）和97.90%（RSD=1.7%）。结论本方法快速简便,精密度高,稳定性和重复性好,可用于鱼腥草芩蓝合剂中绿原酸、黄芩苷和黄芩素3种成分的定量分析和药品质量控制。

OBJECTIVE To establish a simultaneous determination method for paeoniflorin, salvianolic acid B and hydroxysafflor yellow A in Guanxinkang granules. METHODS The determination was performed on a Welchrom C18column（250 mm×4.6 mm, 5 μm） with acetonitrile（A）-0.1% phosphoric acid（B） as the mobile phase in gradient elution mode at the flow rate of 1.0 m L·min^-1. The detection wavelength was 230 nm. RESULTS Paeoniflor presented a good linearity in the concentration range of 7.02-52.64 μg·m L^-1. The average rate of recovery was 99.3 % with RSD of 1.2%. Salvianolic acid B presented a good linearity in the concentration range of 23.19-173.90 μg·m L^-1. The average rate of recovery was 98.2% with RSD of 1.1%. Hydroxysafflor yellow A presented a good linearity in the concentration range of 4.47-33.50 μg·m L^-1. The average rate of recovery was 98.0% with RSD of 1.0%. CONCLUSION This method was simple, accurate and reproducible, it can be used as the basis for quality control of Guanxinkang granules.