摘要
目的建立HPLC测定鱼腥草芩蓝合剂中绿原酸、黄芩苷和黄芩素的含量。方法采用KromasiL^-100 C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.2%磷酸梯度洗脱,流速为1.0 m L·min^-1,检测波长为322 nm,柱温为35℃。结果绿原酸、黄芩苷和黄芩素线性范围分别为0.022 4-1.12μg(R2=0.999 7),0.045 8-2.29μg(R^2=0.999 5)和0.031 8-1.59μg(R2=0.999 9);3种成分的平均回收率分别为99.45%(RSD=1.4%),99.37%(RSD=2.0%)和97.90%(RSD=1.7%)。结论本方法快速简便,精密度高,稳定性和重复性好,可用于鱼腥草芩蓝合剂中绿原酸、黄芩苷和黄芩素3种成分的定量分析和药品质量控制。
OBJECTIVE To establish a simultaneous determination method for paeoniflorin, salvianolic acid B and hydroxysafflor yellow A in Guanxinkang granules. METHODS The determination was performed on a Welchrom C18column(250 mm×4.6 mm, 5 μm) with acetonitrile(A)-0.1% phosphoric acid(B) as the mobile phase in gradient elution mode at the flow rate of 1.0 m L·min^-1. The detection wavelength was 230 nm. RESULTS Paeoniflor presented a good linearity in the concentration range of 7.02-52.64 μg·m L^-1. The average rate of recovery was 99.3 % with RSD of 1.2%. Salvianolic acid B presented a good linearity in the concentration range of 23.19-173.90 μg·m L^-1. The average rate of recovery was 98.2% with RSD of 1.1%. Hydroxysafflor yellow A presented a good linearity in the concentration range of 4.47-33.50 μg·m L^-1. The average rate of recovery was 98.0% with RSD of 1.0%. CONCLUSION This method was simple, accurate and reproducible, it can be used as the basis for quality control of Guanxinkang granules.
出处
《中国现代应用药学》
CAS
CSCD
2015年第5期589-591,共3页
Chinese Journal of Modern Applied Pharmacy