温度和盐度对华贵栉孔扇贝幼贝淀粉酶和Na^+/K^+-ATP酶活性的联合效应

Synergistic Effects of Temperature and Salinity on Activities of Amylase and Na^+/K^+-ATPase in Juvenile Scallop Chlamys nobilis(Reeve)

采用中心复合设计和响应曲面法,研究了温度(19~31℃)和盐度(22~38)对华贵栉孔扇贝幼贝淀粉酶和Na+/K+-ATP酶活力的联合效应。试验结果表明,温度对淀粉酶和Na+/K+-ATP酶活力的一次效应和二次效应影响显著(P<0.05);盐度对Na+/K+-ATP酶活力的一次效应影响显著(P<0.05),但对淀粉酶活力影响不显著(P>0.05);盐度对淀粉酶和Na+/K+-ATP酶活力的二次效应影响显著(P<0.05)。温度和盐度对淀粉酶活力影响存在互作效应,且影响显著(P<0.05),但温度和盐度对Na+/K+-ATP酶活力影响不存在互作效应。温度和盐度过高时,抑制Na+/K+-ATP酶和淀粉酶的活力。采用响应曲面法建立了温度和盐度对淀粉酶和Na+/K+-ATP酶活力影响的模型方程,决定系数分别为0.9815和0.9582,表明模型的拟合度极高,可用于预测华贵栉孔扇贝幼贝中淀粉酶和Na+/K+-ATP酶活力的变化。通过模型优化和验证试验表明:25.60℃和盐度30.67时,淀粉酶和Na+/K+-ATP酶活力达最大值,分别为0.4809U/mg和0.0382U/mg,满意度为0.910。

The synergistic effects of temperature（19—31℃）and salinity（22—38）on activities of amylase and Na＋/K＋-ATPase were studied in juvenile scallop Chlamys nobilis with shell length of（7.52±0.12）mm by two-factor central composite design（CCD）and response surface methodology（RSM）.There were significant linear and quadratic effects of temperature on the activities of amylase and Na＋/K＋-ATPase（P〈0.05）.The significant linear effect of salinity on the activity of Na＋/K＋-ATPase was found（P 〈0.05）,without significant linear effect of salinity on the activity of amylase（P〉0.05）,and the significant quadratic effects of salinity on the activities of amylase and Na＋/K＋-ATPase were observed（P〈0.05）.There were significant synergistic effects of both temperature and salinity on the activity of amylase（P〈0.05）,and no significant synergistic effects of both temperature and salinity on the activity of Na＋/K＋-ATPase were observed.The activities of amylase and Na＋/K＋-ATPase were inhibited under high temperature and salinity,with gradual decline in activities of amylase and Na＋/K＋-ATPase.A model equation about the relationship between the activities of amylase and Na＋/K＋-ATPase and the two factors was established by response surface methodology,with the r2 as high as 0.9815 in temperature and 0.9582 in salinity,indicating that the satisfactory fitting capability of the model was practicably applied for prediction.The optimization of the reliable model revealed that the maximum activities of amylase（0.4809U/mg）and Na＋/K＋-ATPase（0.0382U/mg）,with the desirability value of 0.910,were observed at the2-factor combination of water temperature of 25.60 ℃ and a salinity of 30.67.