微波消解ICP-MS法测定石油污染对虾体内16种元素含量的影响

Effects of Petroleum Pollution on 16 Metallic Element Contents in Shrimp by a Microwave Digestion ICP-MS Method

采用电感耦合等离子体质谱法测定石油污染水体和非石油污染水体虾16种元素的含量。样品经微波消解,通过在线加入内标校正基体效应和接口效应,以修正方程校正质量数干扰,测定元素校正曲线的相关系数均在0.9994以上,方法检出限小于1.83μg/L,精密度优于5.98%,加标回收率为80.5%~119.8%。结果显示,石油污染影响到虾体内多种元素的含量,石油污染水体虾的Cr、Mn、Fe、Co、Ni、Zn、Cu、As、Se、Cd、Pb含量均明显高于非石油污染水体虾,差异均有统计学意义（P〈0.05）。石油污染水体虾的Mg、Al、Ca、Ag、Hg与非石油污染水体虾比较,差异均无统计学意义（P〉0.05）。同时也表明,电感耦合等离子体质谱法适于海产品中多种元素的检测,可以为海产品的质量控制和安全评价提供科学依据。

The inductively coupled plasma mass spectrometry（ICP-MS）method was used for determination of 16 element contents in shrimp in petroleum contaminated waters and non-petroleum contaminated waters.The samples were digested by microwave digestion,through online adding the internal calibration of matrix effect and interface effect,to correct equation and mass disturbance,with the correlation coefficient of above 0.9994 in a determined elements calibration curve,the detection limit of less than 1.83μg/L,the precision of over 5.98%,and the recovery rate from 80.5% to 119.8%.The results showed that the contents of elements in the shrimp were affected by the petroleum pollution.There were significantly higher contents of Cr,Mn,Fe,Co,Ni,Zn,Cu,As,Se,Cd,and Pb in the shrimp in the oil polluted waters than those in the shrimp in the non-oil polluted waters（P 〈0.05）.Compared with the shrimp in the non-oil polluted waters,the shrimp in the oil polluted waters had significantly different contents of Mg,Al,Ca,Ag,and Hg（P〉 0.05）.The findings indicate that the ICP-MS method is suitable for detection of multiple elements in seafood,and provide scientific basis for seafood quality control and safety assessment.