苯系物对仿刺参肠、呼吸树谷胱甘肽过氧化物酶基因表达的影响

Effects of BTEXs on Expression of Glutathione Peroxidase Gene in Intestine and Respiratory Tree of Sea Cucumber Apostichopus japonicus

选择我国黄、渤海重要底栖生物—仿刺参幼参为受试生物,采用实时定量PCR方法,检测6种苯系物（苯、甲苯、乙基苯、邻—二甲苯、间—二甲苯、对—二甲苯）对仿刺参肠、呼吸树两种组织中谷胱甘肽过氧化物酶基因相对表达量的影响。结果表明,6种苯系物造成仿刺参呼吸树、肠组织中谷胱甘肽过氧化物酶基因相对表达量显著变化,并具有显著的剂量—效应关系。其中,苯、邻—二甲苯、对—二甲苯导致呼吸树中谷胱甘肽过氧化物酶基因mRNA表达极显著下调,下调倍数为2.6~41.7;甲苯、间—二甲苯导致呼吸树中谷胱甘肽过氧化物酶基因mRNA表达极显著上调,上调倍数为2.6~86.4。邻—二甲苯、间—二甲苯、对—二甲苯处理组肠谷胱甘肽过氧化物酶基因mRNA表达极显著下调,下调倍数为1.2~38.5。6种苯系物对仿刺参具有氧化胁迫作用。

The present study is aimed to evaluate the effects of six kinds of BTEXs（benzene,toluene,ethyl benzene,o-xylene,m-xylene and p-xylene）on the relative expression levels of glutathione peroxidase（GPx）gene in the respiratory tree and intestine of sea cucumber Apostichopus japonicus,which is an important benthic organism in the Yellow Sea and the Bohai Sea of China,using quantitative real-time PCR method.The results showed that the relative expression levels of GPx gene in respiratory tree and intestine were remarkably changed in different BTEXs treatment groups,and showed dose-effect relationship.The relative expression levels of GPx gene in respiratory tree was down-regulated in the benzene,o-xylene and p-xylene treatment groups,from 2.6-fold to 41.7-fold;and up-regulated in the toluene and m-xylene treatment groups,from 2.6-fold to 86.4-fold.The relative expression levels of GPx gene in intestine was down-regulated in the o-xylene,m-xylene and p-xylene treatment groups,from 1.2-fold to 38.5-fold.This suggested that the BTEXs had oxidative stress on the respiratory tree and intestine of the sea cucumber,and might have oxidative damage in the two tissues.