心肌肥厚大鼠IL-6、TNF-а的表达变化及牛磺酸的干预作用

The significance of tumor necrosis factor-α and interleukin-6 in experiment of myocardium hypertrophy

目的:探讨肿瘤坏死因子-а(TNF-а)、白介素-6(IL-6)在心肌肥厚大鼠血清中的表达及牛磺酸对心肌肥厚的影响和可能机制。方法:Wistar大鼠30只,随机分成3组:对照组、心肌肥厚组、干预组。采用腹主动脉部分缩窄法制作压力负荷性心肌肥厚大鼠模型,饲养4周后采用放射性免疫法检测大鼠血清TNF-а、IL-6水平。结果:与对照组相比,心肌肥厚组血清中TNF-а、IL-6水平升高(P<0.05);与心肌肥厚组相比,干预组SBP、LVW/BW、血清TNF-α、IL-6水平均降低(P<0.05)。结论:TNF-α、IL-6参与心肌肥厚的发生和发展过程。牛磺酸可通过降低心脏后负荷及TNF-α、IL-6的水平,抑制心肌肥厚的发生发展。

Objective：To study the expression of tumor necrosis factor-α（TNF-α）、interleukin-6（IL-6）in serum and in the hypertrophic myocardium of rat Methods：20Wistar rats were divided into 3 groups and one group contained 10 rats. The first group was myocardial hypertrophic group. The second was control group The partial abdominal aorta ligature was used to induce hypertrophy in rats. After 4 weeks, they were killed and cardiac specimen were obtained. TNF-α and IL-6 were determined with radio immunological assay. Results： comparing with control group , The level of TNF-α、IL-6 increased greatly in rats with ventricular hypertrophy （P〈0.05）. Constrast to partial abdominal arota constriction group ,SBP and LVW/BW of partial abdominal arota constriction＋taurine-treated group were decreased, plasma TNF-α and IL-6 levels were decreased （P〈0.05） Conclusion： TNF-α、IL-6 plays a role in myocardial hypertrophy pathogenesis. Taurine can produce a good preventive effect on myocardial hyoertrophy by decreasing pressure overload of heart and plasma TNF-αand IL-6 levels.