医用猪真皮的脱毛及碱处理工艺的设计与优化

Design and Optimization of Unhairing and Alkali Treatment Process about Medical Dermal Pigskin

以可溯源性猪皮为原料,设计7组不同的工艺对猪皮进行纯化处理,通过观测体积变化、组织学观察,对猪皮内胶原纤维的变化情况进行分析处理;通过检测分析废液中羟脯氨酸及总氮含量表征皮胶原的破坏情况,优化出猪皮的最佳脱毛碱处理工艺为滚酶脱毛-碱处理。研究表明:观测体积变化和组织学观察发现,处理过程中胶原纤维得到了充分地分离和松散,纯化猪真皮中不存在细胞及非纤维成分,有利于纯化猪真皮的医学应用。废液中胶原含量和非胶原蛋白质含量的变化表明,最佳纯化工艺在充分松散胶原纤维的同时,皮内的胶原成分得到较为完整的保留。

In this study, seven different processes were designed to purify the traceability pigskin. During the optimization process, the change of skin volume, histological observation,collagen amount and non-collagen protein amount in effluent were examined. Finally, it was been revealed the optimum scheme was roll enzymatic unhairing-alkali treatment program. The study shows that：the collagen fibers are separated well, the cells and the non-fiber component in fresh pigskin are removed completely by hematoxylin-eosin staining after the treatment process, which is benefit to apply in the medical field. The change of collagen amount and non-collagen protein amount in effluent indicates the optimum scheme not only loosed collagen fibers well, but also relatively kept the collagen component perfect.