Preliminary Studies on the Tissue Culture of Cannabis sativa L.(Industrial hemp)

汉麻组织培养的初步研究（英文）

The internodes of the new cultivar Long-ma No.1 of Cannabis sativa L.（Industrial hemp） were used as explants for tissue culture. The paper studied the key factors of industrial hemp tissue culture, such as the physiological state of aseptic seedlings, the selection and concentration of plant growth regulators and so on.Hemp seed disinfection used 75% alcohol for 2 min and sterilized in 1‰ Hg Cl2 for 5min. The best combinations of plant growth regulators were 1.0 mg/L 6-BA and 0.5mg/L NAA for the induction of callus, and the best combinations of hormones were1.0 mg/L KT and 0.5 mg/L NAA for differentiation rate of adventitious bud.

以汉麻新品种"龙麻一号"的茎节为初始材料,研究了汉麻组织培养中的关键因素如无菌苗的获得及其生理状态、植物生长调节剂的选择及其浓度等。选用MS作为基本培养基;种子消毒用75%乙醇浸泡2 min后,再用1‰的升汞溶液(Hg Cl2)浸泡处理5 min;愈伤组织诱导的最适植物生长调节剂组合为1.0mg/L 6-BA和0.5mg/L NAA;不定芽分化的最适激素组合为1.0 mg/L KT和0.5 mg/L NAA。