Study on the Redifferentiation Technology after the Dedifferentiation of Blades of Camellia oleifera Abel.

油茶叶片脱分化后的再分化技术研究（英文）

Based on selecting MS minimal medium, through changing the concentrations and additive amounts of two plant growth regulators of NAA and BA, the redifferentiation experiments of plant morphology on callus and embryos cell which got after the dedifferentiation of ＂Xianglin No.1＂ Camellia oleifera Abel. were carried out.The experimental results showed that callus lines with white, yellowish white or oyster colors, obvious uneven surface and loose texture were inoculated on the medium of MS ＋NAA（0.3 mg/L） ＋BA（2.0 mg/L） ＋saccharose（30 g/L） ＋agar（7 g/L）for 30 d,then multiple shoots were differentiated from the white protuberant part, moreover,the growth vigor was good. If inoculating using regeneration buds of C. oleifera, its multiplication coefficient was 6.50.

在选定了MS基本培养基的基础上,通过改变NAA与BA两种植物生长调节剂的浓度与添加量,展开了针对"湘林1号"油茶叶片脱分化后得到的愈伤组织或胚状体细胞进行的植株形态再分化试验。试验结果表明:将呈白色、黄白色或浅黄绿色、凹凸不平、质地疏松的愈伤组织块接种在MS+NAA(0.3 mg/L)+BA(2.0 mg/L)+蔗糖(30 g/L)+琼脂(7 g/L)培养基中30 d后,在白色突起部位分化出了丛生芽且生长良好。利用油茶的再生芽接种时,其增殖系数为6.50。