重组人骨保护素对牙周炎大鼠牙槽骨RANKL、OPG蛋白表达的影响

Effect of recombinant human osteoprotegerin on RANKL,OPG protein expression in alveolar bone tissue of rat with periodontitis

目的探讨重组人骨保护素(rhOPG)对牙周炎大鼠牙槽骨RANKL、OPG蛋白表达的影响,为rhOPG应用于牙周炎的治疗提供实验依据。方法选择22只Wistar大鼠,采用随机数字表法选择2只健康大鼠作为健康组;其余20只作为实验组,建立牙周炎大鼠模型,再将其分为实验对照组(n=10)和rhOPG组(n=10),rhOPG组大鼠于上颌第2磨牙牙周袋间隙局部注入10mg/kg rhOPG治疗。实验对照组大鼠于相同部位注射等体积灭菌注射用水。采用链霉素抗生物素蛋白-过氧化物酶(SP)检测牙槽骨RANKL、OPG蛋白的表达。结果与健康组比较,实验组大鼠牙槽骨OPG表达水平较低,差异有统计学意义(P<0.05),而RANKL表达水平两组差异无统计学意义(P>0.05)。与实验对照组比较,rhOPG组大鼠牙槽骨组织OPG蛋白的表达水平明显上调,而RANKL蛋白的表达明显下调(P<0.05)。治疗后rhOPG组大鼠牙槽骨中OPG表达水平明显高于治疗前,而RANKL表达水平明显低于治疗前,差异有统计学意义(P<0.05)。结论 rhOPG能调节牙周炎大鼠牙槽骨RANKL、OPG的表达。

Objective To investigate the effects of recombinant human osteoprotegerin（rhOPG） on RANKL,OPG protein expression in alveolar bone tissue of rats with periodonfitis to provide the experimental evidence for the application of rhOPG in periodontitis treatment. Methods Totally 22 Wistar rats were enrolled. The random number table was adopted to select two healthy rats as the healthy group. The rest 20 rats were selected as the experimental group for establishing the rat models of periodontitis, and then subdivided into the experimental control group （n=10） and rhOPG group （n= 10）. Rats in the rhOPG group were locally injected by rhOPG 10 mg/kg at periodontal pocket gap of maxillary second molar, while those in the experimental control group were injected by sterile water for injection at the same site and some volume. The streptavidin-perosidase（SP） method was employed to detect the expression of RANKL, OPG protein in alveolar bone tissue. Results Compared with the healthy group, the expression levels of OPG in alveolar bone tissue of rats in the experimental group were lower with statistically significant difference （P〈0. 05） ,while the difference of RANKL expression levels between the two groups showed no statistical significance（P〉0.05）. Compared with the experimental control group, the expression level of OPG protein in alveolar bone tissue of rats in the rhOPG group was significantly up-regulated,while that of RANKL protein was significantly down-regulated（P〈0.05）. The OPG expres- sion level after treatment in the rhOPG group was markedly enhanced, while the RANKL expression level was reduced compared with before treatment,the difference was statistically significant （P〈0. 05）. Conclusion rhOPG may regulates the expression of RANKL and OPG in alveolar bone tissue of rats with periodontitis.