超高效液相色谱法测定利拉鲁肽原料药的含量

Determination of liraglutide by UPLC

建立了快速、准确、灵敏的测定利拉鲁肽原料药的方法。采用Waters ACQUITY Ultra Performance LC系统(UPLC),Acquity UPLC BEH C18色谱柱(1.7μm,2.1 mm×50 mm),以乙腈-10 mmol/L NH4H2PO4为流动相梯度洗脱,流速为0.21 m L/min,柱温为35℃,检测波长为215 nm。结果表明:该方法高效、快速、灵敏,能很好地测量利拉鲁肽原料药含量。

Ultra performance liquid chromatography （UPLC） is used to determine the liraglutide active pharmaceutical ingredient in this study,. The operation parameters are shown as follows： Waters ACQUITY UPLC system, Acquity UPLC BEH C18 column （1.7 μm,2. 1 mm× 50 mm）, acetonitrile-NH4 H2 PO4 as mobile phase ,0. 21 mL/ rain of flow rate, 35℃ of column temperature, 215 nm of detector wavelength. This rapid, accurate method with high sensitivity provides a good choice for determination of liraglutide.