浓香型大曲中多株芽孢杆菌的分离及鉴定

Separation and Identification of Bacillus Spp. From Luzhou-flavor Daqu

采用传统微生物分离方法从浓香型白酒大曲中筛选出8株优势芽孢杆菌,通过形态特征观察并结合细菌脂肪酸鉴定技术(MIDI鉴定系统)对其进行鉴定。结果表明,从大曲中分离筛选得到8株芽孢杆菌分属于5个种,分别为巨大芽孢杆菌(Bacillus megaterium)、浸麻类芽孢杆菌(Paenibacillus macerans)、短小芽孢杆菌(Bacillus pumilus)、萎缩芽孢杆菌(Bacillus atrophaeus)和地衣芽孢杆菌(Bacillus licheniformis)。

8 dominant Bacillus in Luzhou-flavor liquor Daqu were separated out by traditional microbial separation meth- ods. According to the morphological observation and bacterial fatty-acid identification technique （MIDI identification sys- tem）, 8 dominant Bacillus slap. were identified. The result showed that the 8 dominant bacillus strains were categorized into 5 species （Bacillus megaterium, Paenibacillus macerans, B. pumilus, B. atrophaeus, B. licheniformis）.