丙泊酚和瑞芬太尼联合应用对兔小肠系膜微循环的影响

Effect of propofol combined with remifentanil on rabbit mesenteric microcirculation of rabbit

目的:探讨丙泊酚和瑞芬太尼联合应用对兔小肠系膜微循环的影响。方法:将30只新西兰大白兔随机分为对照组(C组),丙泊酚组(P组),丙泊酚联合瑞芬太尼组(P+R组),每组10只。建立兔全麻模型后,C组持续泵入生理盐水,P组静脉持续泵入丙泊酚,P+R组静脉持续泵入瑞芬太尼和丙泊酚,分别记录3组给药前及给药0.5 h后新西兰大白兔的心率(HR)、平均动脉压(MAP),采用旁流暗视野技术测定新西兰大白兔小肠系膜微循环总血管密度(TVD)、灌注血管密度(PVD)、灌注血管比例(PPV)、微血管流动指数(MFI)。结果:与C组相比,给药后P组HR减慢,MAP轻度下降,微循环血流明显增加,TVD、PVD、PPV、MFI均有所增加,差异均有统计学意义(P<0.05)。与C组相比,给药后P+R组HR减慢,MAP显著下降,微循环血流明显减少,且差异均有统计学意义(P<0.05)。结论:丙泊酚对兔小肠系膜循环有改善作用,丙泊酚和瑞芬太尼联合应用对兔小肠系膜微循环有抑制作用。

Objective： To investigate the effects of combination of propofol and remifentanil on mesenteric microcirculation in rabbits.Methods： Thirty New Zealand white rabbits were randomly divided into control group（group C）, propofol group（P group） and propofolremifentanil combination group（P＋R group） with 10 rabbits in each group. Animal model was established given general anestyesia and 0.9%normal saline solution was infused in C group while propofol was infused in P group. Propofol combined with remifentanil administrated in P＋R group. Multiple parameters of the rabbits in 3 groups were recorded before and half an hour after the administration of drugs, including heart rate（HR）, mean arterial pressure（MAP）, total vessel density（TVD） of mesenteric microcirculation by sidestream dark-field（SDF）technique, perfused vessel density（PVD）, proportion of perfused vessels（PPV） and microvascular flow index（MFI）. Results： Compared with group C, after administration of propofol（P group）, HR in the group dropped, MAP decreased slightly, and blood flow of microcirculation increased significantly（P〈0.05）. In P＋R group, HR and MAP decreased significantly with reduced blood flow in microcirculation, and the differences were statistically significant（P〈0.05）. No significant change of microcirculation parameters was found before and after normal saline solution administration in Group C. The microcirculation was enhanced after propofol infusion in the P group（P〈0.05）. After the administration of propofol and remifentanil, microcirculation for P ＋R group was significantly inhibited（P〈0.05）. Conclusion： Propofol improves mesenteric circulation while a combination of propofol and remifentanil inhibits mesenteric microcirculation in rabbit model.