3种不同方法形成的沉香药材色酮含量测定

Determination of Chromones in Agarwood Formed by Three Different Stimulating Methods

目的建立沉香药材中色酮类成分含量测定方法,考察不同白木香结香方法的药材质量,为评价沉香质量提供实验依据。方法采用可见分光光度法（VS）以磷钨酸钼-硫酸锂为显色剂,以Aquilarone E色酮作为对照品溶液,在760 nm波长下测定沉香药材试液中沉香总色酮的含量。HPLC法测定沉香5种色酮含量,采用Waters Symmetry Shield C18色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈（A）-0.1%磷酸水（B）,流速1m L·min-1,检测波长为252 nm,柱温30℃。结果所建立的可见分光光度法在5 h内稳定性良好,加样回收率为96.71%（RSD=2.12%）。HPLC法测定5种色酮含量在进样量0.1432~7.16、0.00884~0.442、0.01536~0.768、0.01264~0.632、0.0154~0.77μg范围内与峰面积积分值呈良好线性关系。5种色酮平均加样回收率分别为96.98%、97.19%、96.19%、96.52%及95.69%。不同批次沉香含量差异显著,与自然结香组（对照）比较,差异有统计学意义（P〈0.05,P〈0.01）,人工诱导组白木香的结香质量优于传统火烫法,与结香周期较长的自然结香组相近。结论两种检测方法均简便、快速、准确,重复性好,可用于沉香中色酮类成分含量测定。人工诱导白木香快速结香可作为进一步开发利用其资源的重要方法。

Objective To establish methods for the determination of chromones in agarwood and to evaluate the quality of agarwood formed by different stimulating methods,thus to provide evidence for agarwood quality control. Methods The determination of total chromones from agarwood was achieved by ultraviolet spectrometer（VS）,with Auilarone E as s reference substance and 760 nm as detective wavelength. HPLC analysis was used for the determination of 5chromones. The chromatographic conditions were as follows：Waters Symmetry Shield C18Column（4.6 mm×250 mm,5 μm）, a mobile phase of acetonitrile（A）-0.1 % phosphoric acid, flow rate being 1 m L·min-1, the detective wavelength set at 252 nm and the temperature being 30 ℃. Results The VS method was stable within 5 h and the recovery rate was 96.71 %（RSD=2.12 %）. The linear range of HPLC for determination of 5 chromones was 0.1432~7.16,0.00884~0.442,0.01536~0.768,0.01264~0.632,0.0154~0.77 μg,respectively,and the recovery rate was96.98 %, 97.19 %, 96.19 %, 96.52 % and 95.69 % accordingly. The quality of samples formed by artificial indcution method was higher than that of the traditionally fire-scalding method,which was also close to that of the natural agarwood. Conclusion The established two methods are simple,fast,accurate and stable for the determination of chromones in agarwood. The artificial induction method can be a better option for the development and utilization of agarwood.