不同糖皮质激素受体亚型在血管瘤患儿瘤组织中的表达与糖皮质激素治疗效果的相关性

Correlation between expressions of different glucocorticoid receptors subtypes in tumor tissue of children with hemangioma and curative effect of glucocorticoid

目的：探讨不同糖皮质激素受体（GR）亚型α、β的蛋白及mRNA在血管瘤患儿瘤组织中水平与糖皮质激素治疗血管瘤疗效的相关性。方法：选取2013年2月～2015年1月来医院就诊并同意接受糖皮质激素治疗的血管瘤患儿56例作为研究对象,治疗前、后均细针穿刺取血管瘤组织,根据治疗效果分为激素治疗有效组和激素治疗无效组。采用酶联免疫法和实时荧光定量法检测两组患儿瘤组织中GRα、GRβ蛋白及mRNA水平。结果：激素治疗有效组患儿瘤组织中GRα蛋白及mRNA水平在治疗前明显高于激素治疗无效组,两组比较差异有统计学意义（P〈0.01）;GRβ蛋白及mRNA水平在治疗前与激素治疗无效组比较差异无统计学意义（P〉0.05）。结论：糖皮质激素治疗血管瘤患儿的疗效与GRα蛋白及mRNA表达水平呈正相关性,与GRβ蛋白及mRNA表达水平无明显相关性,其治疗机制有待于进一步探讨。

Objective： To explore the correlation between the levels of glucocorticoid receptors （GRs） subtypes α, β proteins and mRNA in tumor tissue of children with hemangioma and curative effect of glucocorticoid. Methods： Fifty-six children with hemangioma and treated with glucocorticoid in the hospital from February 2013 to January 2015 were selected as study objects, fine needle aspiration of he- mangioma was conducted before and after treatment, then the children were divided into effective hormone therapy group and ineffective hor- mone therapy group according to the curative effect. ELISA and real-time fluorescence quantitative method were used to detect the levels of GRoα, GRβ proteins and mRNA. Results： Before treatment, GRα protein and mRNA levels in effective hormone therapy group were statisti- cally significantly higher than those in ineffective hormone therapy group （ P〈0. 01 ） ; there was no statistically significant difference in GRβ protein and mRNA levels between effective hormone therapy group and ineffective hormone therapy group （P〉0. 05 ） . Conclusion： The curative effect of glucocorticoid in treatment of hemangioma is positively correlated with GR α protein and mRNA levels, but the curative effect of glucocorticoid in treatment of hemangioma is not correlated with GR β protein and mRNA, the therapeutic mechanism need to be fur- ther discussed.