山豆根和苦参生物碱类成分UPLC/Q-TOF MS^E比较研究

Comparative Analysis of alkaloids in Radix Sophorae Tonkinensis and Radix Sophorae Flavescentis using UPLC / Q-TOF MS^E

使用UPLC/Q-TOF MSE对山豆根和苦参的生物碱类成分进行详细的比较研究。色谱柱:Acquity UPLC BEH C18(2.1 mm×150 mm,1.7μm);流动相:乙腈-5 mmol/L甲酸铵水溶液;流速为0.5 m L/min;检测器:四极杆串联飞行时间质谱(Q-TOF),正离子检测模式。根据离子流色谱图中色谱峰的分子量及其二级质谱碎片离子的裂解规律,结合参考文献进行结构推定;利用主成分分析及正交偏最小二乘法进行聚类分析。结果从山豆根总生物碱中鉴定12个主要成分,从苦参生物碱中鉴定了11个主要成分,其中有10个是相同的结构,但相同成分在两种药材中的含量差异很大;聚类分析表明山豆根和苦参具有显著的差异。本研究表明山豆根和苦参的主要生物碱成分虽然结构相同,但含量差异很大,二者临床疗效差异,可能由于生物碱含量的差异,或生物碱以外的其他成分所引起。

The present study aimed to compare the alkaloids of Radix Sophorae Tonkinensis and Radix Sophorae Flavescens using UPLC / Q-TOF MS^E. The analysis procedure was carried on an Acquity UPLC BEH C18 column by employing Acetonitrile-5 mmol / L ammonium formate aqueous solution at a flow rate of 0. 5 m L / min,and quadrupole tandem timeof-flight mass spectrometry（ Q-TOF） in positive ion mode was used as detector. Chemical structures of alkaloids were tentatively identified by the molecular weight of ion chromatographic peak and its characteristic fragmentations combined with related references. In addition,principal component analysis and orthogonal partial least-squares analysis were employed for cluster analysis. The results showed that 12 and 11 alkaloids were identified from Radix Sophorae Tonkinensis and Radix Sophorae Flavescens,respectively; 10 of which were identical,but the contents of these 10 identical compounds differed greatly between Radix sophorae Tonkinensis and Radix Sophorae Flavescens,and cluster analysis also revealed that Radix Sophorae Tonkinensis was significant different from Radix Sophorae Flavescens. The present study indicated that the category of alkaloids in Radix Sophorae Tonkinensis and Radix Sophorae Flavescens were similar but their contents were of great difference. The different clinic curative effects between Radix Sophorae Tonkinensis and Radix Sophorae Flavescens may be due to the content differences among alkaloid,or other components besides alkaloids.