河南和山西连翘叶中总木脂素、连翘酯苷A、连翘酯苷B和连翘苷的含量比较

Determination of Total Lignans,Forsythiaside A,Forsythiaside B and Phillyrin in Forsythia suspensa Leaves from Henan and Shanxi

测定河南和山西连翘叶中总木脂素、连翘酯苷A、连翘酯苷B和连翘苷含量。采用分光光度法测定连翘叶中总木脂素含量,结果显示山西连翘叶高于河南连翘叶总木脂素含量。采用高效液相色谱法(HPLC)测定连翘叶中连翘酯苷A、连翘酯苷B和连翘苷的含量。连翘酯苷A平均回收率为99.6%,RSD为1.23%;连翘酯苷B平均回收率为101.3%,RSD为1.74%;连翘苷平均回收率为102.3%,RSD为2.58%;连翘叶中连翘酯苷A和连翘苷的含量较高,并且不同产地连翘叶中连翘苷含量差异较大;另外对于富含连翘酯苷和连翘苷的连翘叶能否代替果实或者与果实合并用药有待于进一步研究。

The objective of this study was to determine the content of total lignans,forsythiaside A,forsythiaside B and phillyrin in Forsythia suspensa leaves from Henan province and Shanxi province. The total lignans of F. suspensa leaves from Shanxi province were higher than Henan province by spectrophotometry method. The contents of forsythiaside A,forsythiaside B and phillyrin were determined by HPLC. The average recoveries of forsythiaside A,forsythiaside B and phillyrin were 99. 6%（ RSD = 1. 23%）,101. 3%（ RSD = 1. 74%） and 102. 3%（ RSD = 2. 58%）,respectively. The experimental results showed that the content of forsythiaside A and phillyrin in F. suspensa leaves from Shanxi and Henan was relatively high. In addition,the content of phillyrin in F. suspense leaves from different habitats were of large difference. F. suspensa leaves are rich in forsythiaside and lignans,more researches on F. suspensa leaves are still needed to confirm whether it can be used as substitute or cooperate with F. suspensa fruit for medicinal application.