3种中药提取液对鸡艾美耳球虫卵囊孢子化的影响

Effects of Three Kinds of Traditional Chinese Medicine Extracts on Oocyst Sporulation of Eimeria

为探讨中药提取液对体外球虫卵囊孢子化的影响,将3种不同艾美耳球虫未孢子化卵囊与不同浓度(25%,50%,100%)的中药商陆、白头翁、常山提取液共培养24 h和48 h,显微镜观察球虫卵囊的孢子化情况,计算孢子化率。结果发现各种中药浓度为50%的条件下,24 h后,柔嫩、毒害、堆型艾美耳球虫的孢子化率在商陆培养下分别为26.20%、4.74%、24.09%,在白头翁培养下分别为57.95%、10.74%、20.74%,在常山培养下分别为64.65%、20.53%、18.03%;48 h后,柔嫩、毒害、堆型艾美耳球虫在商陆培养下分别为58.41%、40.89%、31.45%,在白头翁培养下分别为64.81%、19.42%、60.90%,在常山培养下分别为64.18%、39.70%、63.16%。与对照组比较,药物组球虫培养24 h和48 h后,卵囊孢子化率显著降低,100%浓度的药物抑制效果最好,50%浓度药物次之。3种中药提取液对鸡艾美耳球虫卵囊体外孢子化均有一定的抑制作用,且随着药物浓度增加,对球虫孢子化的抑制作用增强;但抑制程度有差异,其中商陆抑制作用最好。

To investigate the effects of traditional Chinese medicine extracts on oocyst sporulation of Eimeria in vitro, un- sporulated oocysts of three species of Eimeria were cultured in vitro with extracts of traditionalChinese medicine which were Phytolacca, Pulsatilla and Dichroa at different concentrations（25%,50%,100%）for 24 hours and 48 hours, respectively. Oocysts were observed with microscope and sporulated rate wascalculated. The results showed that when the concentration of the three kinds of medicines was 50%,sporulated rates of E. tenella, E.necatrix and E.acervulina were 26.20%, 4.74% and 24.09% when cultured in Phytolacca, 57.95%, 10.74% and 20.74% when cultured in Pulsatilla, and 64.65%, 20.53% and 18.03% whencultured in Dichroa after 24 hours. Sporulated rates of E. tenella, E.necatrix and E.acervulina were 58.41%、40.89% and 31.45% when cultured in Phytolacca, 64.81%, 19.42% and 60.90% when cultured in Pulsatilla,64.18%, 39.70% and 63.16% when cultured in Dichroa after 48 hours. There was a significant reduction insporulated rate of oocysts cultured for 24 hours and 48 hours in drug groups compared with that of the controlgroup. There were differences among the three drugs inhibited. Concentration of traditional Chinese medicineextracts being 100% showed the best effect in inhibiting sporulation, then was the concentration of 50%. There were inhibitory effects of the three kinds of traditional Chinese medicine extracts on oocyst sporulation in vitro of Eimeria to a certain extent. Inhibitory effect increased with the increase of drug concentration. Phytolacca showed the best inhibitory effect.