重金属胁迫背景下的任豆幼苗抗性生理试验

Resistance Physiological Experiment of Zenia insignis Seedlings in Heavy Metal Stress Environment

分别用浓度为0～30 mg／L和0～150 mg／L的重金属Cu2＋、Cd2＋、Pd2＋、Zn2＋处理任豆幼苗，观察其生长情况，测定其叶片的叶绿素含量、丙二醛含量及总黄酮含量，分析其抗性生理特征。结果表明：任豆幼苗对不同重金属离子胁迫的耐性不同，在不同浓度的同一重金属离子胁迫下，任豆幼苗叶片的叶绿素、丙二醛、黄酮含量有明显差异；任豆幼苗对不同重金属的抗性不同，对Cu2＋、Cd2＋、Pd2＋、Zn2＋四种重金属离子耐性大小的排序为：Pd2＋＞Zn2＋＞Cd2＋＞Cu2＋。根据任豆幼苗在重金属胁迫下所表现出的抗性生理特征及相关的实验结果，可利用任豆对Pd、Zn、Cd、Cu污染的土壤进行修复。

Using heavy metal Cu2 ＋ , Cd2＋ , Pd2＋ and Zn2＋ with concentration of 0 - 30 mg/L or 0 - 150 mg/L to treat the Zenia insignis seedings respectively, the author observed the growth of Zenia insignis seedings, determi- nation the chlorophyll content, MDA content and total flavonoids content of the leaves, and analyzed the resistance physiological characteristics of Z. insignis seedings. The results showed that Z. insignis seedings had different tol- erance when stressed with different heavy content, total flavonoids content of Z. ins the same heavy metal ion. The resistance Z. insignis seedings to Cu2~ ,Cd2~ ,Pd2~ metal ions. There is significant difference of chlorophyll content, MDA ignis seedings leaves, when it was stressed with different concentration of of Z. insignis seedings to different heavy metal was different, the order of ,Zn2~ was as follows： Pd2 ＋ 〉 Zn2 ＋ 〉 Cd2 ＋ 〉 Cu2＋. Based on the resist- ance physiological characteristics and related results, Z. lead, zine, cadmium and copper pollution. insignis was considered to be used for soil remediation of