亚慢性镉染毒大鼠MALAT1异常表达与肝肾损伤的关系

Relationship of abnormal expression of MALAT1 in rats subchronic exposure to cadmium with the damage of liver and kidney

目的研究肺癌转移相关转录子1(MALAT1)在亚慢性氯化镉(Cd Cl2)染毒大鼠模型肝肾组织中的表达情况,探讨MALAT1与镉致肝肾损伤过程中的关系。方法在已建立的亚慢性Cd Cl2染毒大鼠模型中,采用实时荧光定量反转录PCR(QRT-PCR)技术检测肝肾组织中MALAT1表达情况,分析MALAT1与测定的肝功能生化指标(ALT、AST)、肾功能生化指标(BUN、SCR)、相关基因(FOXC2、BCL-2、BAX、TGF、STAT、EGFR)及肝肾镉含量的相关性。结果与各自的对照组相比,大鼠肝肾组织中MALAT1表达水平均呈不同程度的升高,并有随染毒剂量的增高而升高的趋势,高剂量组表达量分别为(2.63±1.31)和(2.73±0.99),均为对照组的2.46倍,差异均有统计学意义(均P<0.05)。肝脏组织中MALAT1表达与血清中ALT含量存在相关性(r=0.459,P<0.05);肾脏组织中MALAT1表达与肾镉(r=0.427),BUN(r=0.531),SCR(r=0.400)含量及EGFR表达水平(r=0.466)均存在相关关系(均P<0.05)。结论 MALAT1在亚慢性镉染毒大鼠肝肾组织中表达水平增高,且与肝肾功能指标含量存在相关关系。肺癌转移相关转录子1可能在镉致肝肾损伤过程中发挥作用。

[Objective]To research the expression of MALA T1 in liver and kidney tissues of rats Subchronic exposure to cadmium chloride （CdC12）, investigate the relationship of MALAT1 with the damage of liver and kidney by cadmium.[nethods]In the established rats subchronic exposure to CdC12 model, real-time fluorescent quantitative reverse transcriptase polymerase chain reaction （QRT-PCR） were used to detect the expression of MALA T1 in liver and kidney tissues, and the correlation of MALA T1 with biochemical indicators of liver function（ALT, AST）, biochemical indicators of kidney function （BUN, SCR）, related gene （FOXC2, BCL-2, BAX, TGF, STA T and EGFR） and cadmium content in liver and kidney was analyzed.[Results]Compared with the control group, the expression level of MALAT1 in liver and kidney tissues of rats increased, and had a increasing trend with the increasing of exposure doses. The expression quantity in high-dose group were （2.63 ±1.31） and （2.73± 0.99） respectively, all increased 2.46 times compared with the control group, and the difference was statistically significant （P〈0.05）. The expression of MALA T! in liver and ALT in serum were correlation （r=0.459, P〈0.05）; the expression of MALA T1 in kidney and content of kidney cadmium （r=0.427）, BUN （r=0.531 ）, SCR （r=0.400）and EGFR （r=0.466） showed correlation （all P〈0.05 ）. [Conclusion]The expression level of MALAT1 in liver and kidney tissues of rats subchronic exposure to cadmium increased, and has correlativity with the content of liver and kidney function indicators. MALAT1 may play a role in the process of cadmiuminduced liver and kidney damaged.