何首乌提取物对糖尿病肾病早期大鼠肾组织iNOS和NT表达的影响

Effects of Heshouwu(Polygonum Multiflorum) Extract on iNOS and NT Expressions in Renal Tissue of Rats with Early Diabetic Nephropathy

目的:观察何首乌提取物二苯乙烯苷(TSG)对糖尿病肾病(DN)早期大鼠肾组织诱导性一氧化氮合酶(i NOS)和硝基酪氨酸(NT)表达的影响。方法:实验分3组,正常组,模型组、TSG组;采用左侧肾结扎术加尾静脉注射链脲佐菌素(STZ,40 mg/kg)制备DN早期大鼠模型,血糖稳定2周后给予TSG(20mg/kg·bw)腹腔注射干预治疗,6周后,采用HE染色,光镜观察肾脏组织病理形态学变化,电镜观察超微结构的变化;散射比浊法测大鼠24小时尿白蛋白排泄率、尿微量白蛋白/尿肌酐(UAER、MAU/Ucr);免疫组化法、Western-blot观察DN早期大鼠肾组织i NOS、NT蛋白表达的变化。结果:TSG组大鼠肾脏病理形态学变化、超微结构变化明显减轻,i NOS在肾小管上皮细胞表达明显减少,肾脏NT蛋白表达量明显降低,与模型组比较均有统计学意义(P<0.05)。结论:TSG对DN早期大鼠肾脏具有保护作用,其机制与下调i NOS、NT表达,减轻氧化应激损伤有关。

Objective： To study the effects of 2,3,5,4＇ - Tetrahydroxystilbene - 2 - O - β - D - glucoside （TSG） extracted from Polygonum multiflorum on iNOS and NT expressions in renal tissue of rats with early dia- betic nephropathy. Methods：Experiment was divided into three groups;normal, model and TSG groups, early DN model was established by unilateral nephreetomy plus low -dose STZ intraperitoneal injection （40 mg/kg）, blood sugar was stable for 2 weeks and TSG treatment group was given TSG intraperi toneal injection for six weeks. The pathologic changes of renal tissue and ultrostructure were observed by light microscope and electron microscope, immunohistochemical staining was used to observe the expression of iNOS, Western blot analysis was used to de- termine the NT protein expression changes, special protein analyzer analysised UAER and MAU/Ucr. Results： The histologic changes of real tissue in TSG group were significantly alleviated and uhrostucture changes were ob- viously improved,iNos epression in rats＇ renal tissue was reduced significantly, NT protein expression were re- duced, too （P 〈 0. 05 ）. Conclusion：TSG can effectively protect kidney from injury, the mechanism is probably associated with decreasing the protein expressions of iNOS, NT and alleviating the oxidative stress.