光质对火焰无核葡萄胚挽救及试管苗生长发育的影响

Effect of light quality on embryo rescue and test-tube plantlets growth and development of Flame Seedless

以火焰无核自交胚珠为试材,研究了红光、蓝光、白光及暗处理对胚发育、胚萌发及试管苗生长发育的影响。结果表明:3种光质处理对胚发育均有一定促进作用,但与对照未达到显著性水平。在胚萌发阶段,红光对胚萌发有明显的促进作用。在试管苗发育成苗阶段,以蓝光处理的试管苗最为矮壮,红光下葡萄胚挽救苗试管苗节间最长,发育最高,叶绿素含量最低;各处理试管苗继代后移栽成活率均可达到90%以上。

The open pollination ovules from flame Seedless were used to investigate the effect of diffierent light quality on the proportion of embryo development and germination ,and test-tube plantlets growth and development in vitro, including red light, blue light, white light and dark condition. The results showed that three lights had positive effect on embryo during ovule development, but did not significantly; Red light could promote obsively embryo germination; the plantlets were the most stocky under blue light. Red light was favorable to the longitudinal growth of the test-tube Plantlets, Chlorophyll content were the lowest. Each treatment plantlets transplant survival rate could reach 90%.