摘要
目的:研究雪菊总黄酮及其主要成分的抗氧化活性。方法:对雪菊总黄酮的主成分进行分离鉴定和含量测定,采用1,1-二苯基-2-三硝基苯肼(DPPH)和2,2-联氮-二-(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)法测试其抗氧化活性。结果:分离鉴定了雪菊总黄酮中的黄诺玛苷、马里苷2个主成分,雪菊总黄酮对DPPH、ABTS自由基的半数清除率依次为11.06、139.3μg·m L-1,与马里苷、黄诺玛苷的半数清除率相近。结论:雪菊精制总黄酮具有优良的抗氧化能力,其中主要含有马里苷、黄诺玛苷等黄酮类活性成分。
Objective： Antioxidant capacities of the total flavonoid extract of coreopsis tinctoria （CTFE） and its main constituents were investigated in this study. Methods： Key components in CTFE were separated and determined. DPPH and ABTS tests were applied to measure their antioxidant capacities. Results： Marein and flavanomarein were identified as two key components in CTFE. The half inhibition concentrations of CTFE against DPPH and ABTS free radical were 11.06 and 139.3. mL^-1, respectively, similar to those of marein and flavanomarein. Conclusion： CTFE showed strong antioxidant capacities, with marein, flavanomare and other flavonoids as the bioactive constituents.
出处
《中国现代中药》
CAS
2015年第5期435-439,共5页
Modern Chinese Medicine
基金
国家自然基金项目(81460634)
新疆医科大学第一附属医院自然基金项目(2013ZRZD07)
关键词
两色金鸡菊
抗氧化
马里苷
黄诺玛苷
Coreopsis tinctoria
antioxidant
marein
flavanomarein
