摘要
目的：建立同时测定羊藿三七胶囊中朝藿定C和淫羊藿苷含量的高效液相色谱法。方法：采用Agilent C18柱（150 mm×4.6 mm,5μm）;流动相为乙腈-0.05%磷酸溶液（26∶74）;流速为1.0 m L·min^-1;检测波长为270 nm。结果：朝藿定C进样量在0.288-9.198μg、淫羊藿苷进样量在0.111-3.558μg与峰面积呈良好的线性关系（r=0.999 9）;朝藿定C的平均回收率为103.0%,RSD=0.8%（n=6）;淫羊藿苷的平均回收率为98.9%,RSD=1.8%（n=6）。结论：本方法简便、准确,重复性好,可作为羊藿三七胶囊中淫羊藿的含量测定方法。
Objective：To establish a HPLC method for simultaneous determination of epimedin C and icariin in Yanghuo Sanqi Capsules.Methods：The samples were separated on Agilent C18 column（ 150 mm × 4.6 mm,5 μm） by acetonitrile-0.05% phosphoric acid solution（ 26 ∶ 74） as the mobile phase.The flow rate was 1.0 m L·min^- 1and the detection wavelength was 270 nm.Results：The calibration curves showed good linearity in the range of 0.288-9.198 μg（ epimedin C）,0.111-3.558 μg（ icariin）.The average recoverise（ n = 6） were 103.0%（ RSD = 0.8%）,98.9%（ RSD = 1.8%）,respectively.Conclusion：The method is simple and accurate with good reproducibility.It can be used for the determination of Epimedii Folium in Yanghuo Sanqi Capsules.
出处
《中国现代中药》
CAS
2015年第5期485-487,496,共4页
Modern Chinese Medicine
