脉冲电流经皮刺激肝区对运动性疲劳大鼠5-HT及其代谢的影响

Influence of Percutaneous Impulsive Current Stimulation of Hepatic Region on the Concentration of 5-HT and Its Metabolism in Exercise-induced Fatigue Rats

目的:研究脉冲电流经皮刺激肝区对运动性疲劳大鼠大脑纹状体5-羟色胺(5-HT)及其代谢的影响。方法:8周龄Wistar雄性大鼠80只随机分为安静对照组(CG组)、疲劳训练组(FG组)、运动后刺激组(SAF组)、运动前刺激组(SBF组),除CG组外各组均进行游泳训练,建立运动疲劳模型,于第1、3、5周训练的最后1d,断头处死,取外周血测游离色氨酸(F-Trp)、支链氨基酸(BCAA)、F-Trp/BCAA含量,取纹状体测F-Trp、5-HT、5-羟吲哚乙酸(5-HIAA)含量。结果:与CG组相比,各组大鼠血清BCAA含量呈下降趋势,其余各指标均呈升高趋势;与FG组相比,SAF组、SBF组第3周末纹状体5-HT明显下降(P<0.01);与SBF组相比,SAF组第5周末纹状体5-HT明显下降(P<0.05)。结论:经皮脉冲电流刺激具有降低疲劳大鼠5-HT及代谢产物的含量,有助于疲劳的消除,对长时间运动时中枢机能的改善具有积极作用。

Objective： To investigate the effects of percutaneous impulsive current stimulation of hepatic region on the concentration of 5-hydroxytryptamine（5-HT） and its metabolism in striatum of fatigued rats. Methods： 80 Eight-week-year old Wistar male rats were randomly divided into four groups： control group（ CG）, fatigue group（ FG）, stimulation after fatigue group（ SAF） and stimulation before fatigue group（SBF）. All of the groups were given the swimming training to establish exercise-induced fatigue model except for the CG group. Rats were killed immediately, The level of free-tryptophan（F-Trp）, branched chain amino aci（BCAA） and the ratios of F-Trp/BCAA were Measured in blood serum of rats, the concentration of F-Trp, 5-HT and 5-hydroxyindoleacetic acid（5-HIAA） were tested in Striatum of Rats. Results： Compared with CG group, the concentrations of BCAA in blood serum of rats in every group tended to decline,other indexes were all increased. Compared with FG group, the concentrations of 5-HT in rats＇ striatum in SAF group and SBF group declined significantly（P〈0.01） at the weekend of 3rd week. Compared with SBF group, the concentrations of 5-HT in rats＇ striatum in SAF group declined significantly（P〈0.01） at the weekend of 5th week. Conclusions： Percutaneous impulsive current stimulation of hepatic region can effectively lessen the concentration of 5-HT and its metabolism in fatigued rats, which could suspend the occurrence of fatigue and help to eliminate the fatigue,improve rat central nerves performance in long time exhausted exercise.