红芪胶囊对低剂量辐射损伤小鼠的保护作用

The Protective Effect of Hongqi Capsule on Low-dose Radiation Injured Mice

目的:采用低剂量辐射损伤小鼠模型,观察红芪胶囊的药理作用。方法:二级昆明种小鼠216只,除正常组36只外,其余180只采用多次低剂量累积辐射。将照射的180只小鼠按体重随机分为5组,每组12只,分别为:模型组,阳性对照组(1.20 g/kg),红芪胶囊小剂量组(0.175 g/kg),红芪胶囊中剂量组(0.35 g/kg),红芪胶囊大剂量组(0.70 g/kg);将未照射的36只小鼠设为正常组,每组又分为给药6、12、24 d三个时间点,每时间点12只动物。各组在末次照射当天分别灌胃上述药物,正常对照组及模型对照组灌胃等量蒸馏水,每日一次,连续给药24 d,观察红芪胶囊的药理作用。结果:1红芪胶囊给药12 d、24 d对辐射损伤小鼠的外周血象红细胞(RBC)、白细胞(WBC)、血小板(PLT)和血红蛋白含量(HGB)有明显的升高作用(P<0.01或P<0.05);2明显增加辐射损伤小鼠的肝脏、脾脏、大脑和睾丸组织的湿质量(P<0.01或P<0.05);3镜下观察可见红芪胶囊能明显改善损伤小鼠骨髓细胞活性。结论:红芪胶囊对低剂量辐射损伤具有一定保护作用。

Objective： To investigate the pharmacological effects of Hongqi capsule on low-dose radiation injured mice. Methods：216 Kunming mice were divided into normal group and experiment group. There were 36 mice in the normal group, while 180 in the experiment group were irradiated by multiple low-dose radiation, and then they were randomly divided into five groups of 12 each：the model group, the positive control group（1.20g/kg）, Hongqi capsule small dose group（0.175g/kg）, Hongqi capsule middle dose group（0.35g/kg）, and Hongqi capsule high-dose group（0.70g/kg）; 36 non-irradiated mice were divided into the normal group, each group was administrated for 6, 12, 24 days, respectively. Every group was administered the drugs on the day of the last irradiation, and the normal control group and model control group were given distilled water, once-daily. The pharmacological effects of Hongqi capsule after continuous administration of 24 days were detected. Results： 1 Hongqi capsule increased the red blood cell（RBC）, white blood cell（WBC）, platelet（PLT） and hemoglobin content（HGB） of radiation injured mice in peripheral blood after administration for 12 and 24 days.（P〈0.01 or P〈0.05）; 2Hongqi capsule increased the wet weight of liver, spleen, brain and testicular tissue（P〈0.01 or P〈0.05）; 3Hongqi capsule increased improved marrow cell activity in microscope. Conclusions： Hongqi capsule certainly has protective effect on low-dose injured mice.