梅花PmAP2基因的克隆及表达

Cloning and Expression of Floral Organ Identity Determination Gene PmAP2 in Prunus mume

为了解析梅花(Prunus mume Sieb.et Zucc.)花器官发育的分子调控机理,采用RT-PCR的方法从梅花‘三轮玉蝶’中克隆了APETALE2的同源基因Pm AP2,并采用荧光定量PCR对Pm AP2的表达模式进行了分析。序列分析表明,Pm AP2基因CDS区域全长1 647 bp,编码548个氨基酸,含有两个保守的AP2结构域,属于AP2/ERF基因家族。多序列比对和系统进化结果显示,该基因与桃、苹果AP2基因相似性较高,分别为96%和82%。Pm AP2在梅花营养器官、四轮花器官和果实中均有表达,花瓣中的表达量最高;台阁花型梅花花中Pm AP2表达量最高,重瓣品种次之,单瓣品种表达量最低。Pm AP2的差异表达可能与梅花的花型进化有关。

In order to elucidate the molecular mechanism that controls the floral organ development in Prunus mume, RT-PCR was employed to clone the APETALE2 homologous gene PmAP2 from Prunus mume ＇ Sanlun Yudie＇. The expression pat- terns of PmAP2 were determined by real-time RT-PCR. A l 647 bp CDS encoding a protein of 548 amino acids was ob- tained. The CDS contains two conserved AP2 domain and belongs to AP2/ERF gene family. By multiple sequence align- ment and phylogenetie analysis, the eDNA was highly homologous to the AP2 genes from peach and apple, and the homolo- gous identities were 98% and 82%, respectively. The transcript of PmAP2 was detected in vegetative organs, floral organs and fruit with the highest expression level observed in petal. The expression pattern of PmAP2 in the flower of different flower types was also investigated. The duplicated flower type exhibited the highest expression level while the simple type showed the lowest. The distinct expression patterns of PmAP2 in different flower types may contribute to the evolution of flower type in P. mume.