高产碱性蛋白酶解脂耶罗维亚酵母菌选育及培养基优化

Screening of Yarrowia lipolytica with high alkaline protease yield and medium composition optimization

以解脂耶罗维亚酵母(Yarrowia lipolytica)ZJ059为出发菌株,利用紫外线(UV)-氯化锂(Li Cl)复合诱变处理,筛选出遗传性状稳定、高产碱性蛋白酶的突变菌株F053。以碱性蛋白酶产量为指标,对菌株F053进行培养基优化,经过单因素试验及正交优化试验,确定最佳碱性蛋白酶培养基成分为葡萄糖2.0%、蛋白胨0.5%、硫酸钙0.075%;在此优化条件下,碱性蛋白酶产量高达44.39 U/L,与出发菌株相比,碱性蛋白酶产量提高20.46%。

Yarrowia lipolytica ZJ059 was used as original strain to screen genetic stable and high alkaline protease yield mutant strain F053 by UV-Li- Cl compound mutagenesis. Using alkaline protease yield as index, the medium composition for F053 production was optimized by single factor and orthogonal experiment. Results showed the optimal medium composition was glucose 2.0%, peptone 0.5%, and calcium sulfate 0.075%. Under the optimized condition, the yield of alkaline protease was up to 44.39 U/L, 20.46% more than that of the original strain.