滇池金线鲃、西畴金线鲃及其杂交F1代的形态分析

Morphological analysis in Sinocyclocheilus grahami,S. xichouensis and their hybrid F1( S. grahami♀ × S. xichouensis ♂ )

根据传统形态度量学和框架结构形态学法测量了15月龄的杂交F1代(滇池金线鲃Sinocyclocheilus grahami♀×西畴金线鲃S.xichouensis♂)以及滇池金线鲃(S.grahami)和西畴金线鲃(S.xichouensis)的10项传统可量性状数据和24项框架结构数据以及鳍条、侧线鳞和脊椎骨等可数性状数据。并用方差分析和多元统计分析法(聚类分析,判别分析和主成分分析)对三者的传统可量性状和框架结构数据进行分析。结果显示:三者的可数性状差别不大,杂交F1代体表花纹与双亲相比发生一定变化。聚类分析结果表明,杂交F1代的形态与母本滇池金线鲃形态较为相似。采用逐步判别分析方法建立了3种鱼的判别函数,判别准确率较高(90%,90%,100%)。主成分分析结果及主成分散点图显示:三者均有重叠区域不容易从形态上清楚分开,但杂交F1代明显更接近母本滇池金线鲃。结合方差分析和主成份分析发现杂交F1代与双亲不同的性状主要集中在头部和尾部。

10 traditional measurable character data and 24 truss data and countable characters such as number of fin ray , lateral-line scale, vertebrae and so on of Sinocyclocheilus graham, S.xichouensis and their hybrid F1 were collected and analyzed by analysis of variance , cluster analysis, discriminant analysis and principal component analysis .The results showed , there was no significant difference in countable characters of hybrid F 1 and its parents , but surface pattern of hybrid F1 was different with both S.graham and S.xichouensis.Morphological characteristics of hybrid F 1 were more similar with S.graham than S.xichouensis according to cluster analysis .Discriminant functions of hybrid F1and its parents were build based on discriminant analysis , and the discriminant accuracy was comparatively high （90%in hybrid F1, 90%in S.grahami, 100%in S.xichouensis） .Based on the results of principal components analysis , the 3 kinds of fish clustered together by scatter plots of scores could not be divided clearly , however, there was more overlap between hybrid F1 and S.graham.Both analysis of variance and principal components analysis showed the different morphological characteristics between hybrid F1 and its parents were mainly concentrated in the head and tail .