摘要
采用PCR方法,对分离于唐山和秦皇岛养殖场的宽体金线蛭(Whitmania pigra)、中国林蛙(Rana temporaria chensinensis)、牙鲆(Paralichthys olivaceus)、草鱼(Ctenopharyngodon idellua)、青鱼(Mylopharyngodon piceus)以及鲤(Cyprinus carpio)肝脏的42株不同水产养殖动物的致病性嗜水气单胞菌(Aeromonas hydrophila)进行了4种耐药基因β-内酰胺类(TEM)、氨基糖苷类(ant(3″)-I)、磺胺类(Sul3)和四环素类(tet(A))的检测。结果表明:42株嗜水气单胞菌均100%携带TEM、ant(3″)-I和Sul3,不携带tet(A)。所检不同水产动物源嗜水气单胞菌均携带TEM、ant(3″)-I及Sul3耐药基因,从分子层面表明该致病菌多重耐药或潜在耐药严重。
42 pathogenic strains of Aeromonas hydrophila isolated from the liver of Whitmania pigra, Rana chensinensis, Paralichthys olivaceus, Ctenopharyngodon idellua, Mylopharyngodon piceus and Cyprinus carpio cultured in the farms of Tangshan and Qinhuangdao were investigated for the presence of resistance genes -TEM, ant （3″）-Ⅰ, Sul3 and tet（A） using PCR method.The results showed that all detected strains were positive for TEM , ant （3″）-Ⅰand Sul3 gene, and negative for tet （ A） gene.A.hydrophila strains isolated from different aquiculture animals contained multiple resistant genes showed antibiotic resistance or potential resistance of the pathogenic strains was serious .
出处
《淡水渔业》
CSCD
北大核心
2015年第3期84-87,92,共5页
Freshwater Fisheries
基金
河北省自然科学基金项目(2015407074)
现代农业产业技术体系河北省创新团队建设(特色海产品疫病防控与质量安全)
秦皇岛市科学技术研究与发展计划项目(201401A058)
