两种麻醉剂对罗非鱼携氧相关血液指标的影响

Effect of two anesthetics on oxygen-carrying related hematological parameters of Oreochromis niloticus

分别用3个浓度梯度的MS-222和苯唑卡因对尼罗罗非鱼（Oreochromis niloticus）（6.050 0±1.710 3）g进行6 h浸泡式麻醉（水温30.7-32.3℃）,然后抽取尾部静脉血,测定血液生理生化指标。重点分析了与生物携氧功能密切相关的6个指标的变化,结果显示：（1）较高浓度（25 mg/L、40 mg/L）MS-222导致红细胞数目（Er）升高,而苯唑卡因导致该值大幅下降;（2）两种麻醉剂对红细胞平均血红蛋白含量（MCH）都无显著影响,且MS-222处理后,血液的血红蛋白浓度（Hb）也无明显变化,但经苯唑卡因麻醉处理后,Hb显著下降,且降幅随药物浓度升高而显著增大。

Oreochromis niloticus with body weight of （6.0500 ±1.7103 ） g was exposed to MS-222 and benzocaine for 6 hrs in 3 concentration gradients （ with water temperature of 30.7-32.3 ℃） , and blood from caudal vein was abstracted to determine the hematological parameters .The results showed that , the erythrocyte count （ Er） increased while exposed to MS-222 in high concentrations （25 mg/L and 40 mg/L）, however, decreased dramatically while exposed to benzocaine . The mean corpuscular hemoglobin contents （ MCH） of fish exposed to anesthetics did not changed significantly comparing with the control group, so did the hemoglobin concentration （Hb） of fish exposed to MS-222.However, benzocaine generated marked drop in Hb , furthermore, the drop became greater with the increasing of the anesthetic concentration .