侵袭性垂体腺瘤患者临床特征与基质金属蛋白酶9、血管内皮生长因子表达的相关性

Clinical features correlated with MMP-9, VEGF expression in invasive pituitary adenoma patients

目的 探讨侵袭性垂体腺瘤患者临床特征与基质金属蛋白酶9（MMP-9）、血管内皮生长因子（VEGF）表达水平的关系.方法 选取96例垂体腺瘤患者为研究对象,46例侵袭性垂体腺瘤,50例非侵袭性垂体腺瘤,免疫组化法测定瘤组织中MMP-9、VEGF表达水平,分析其与肿瘤侵袭性的相关性.结果 侵袭性垂体腺瘤组织中MMP-9、VEGF强阳性表达率为54.35％、52.17％,总阳性表达率为89.13％、95.65％,显著高于非侵袭性垂体腺瘤组织中MMP-9、VEGF强阳性表达率的16.00％、20.00％,总阳性表达率的44.00％、56.00％（χ2=15.618、9.416、21.631、20.090,均P＜0.05）;MMP-9、VEGF为阳性情况下侵袭性垂体腺瘤体积、最大径显著高于非侵袭性组（t =4.32、3.67、2.49、2.54,均P＜0.05）,MMP-9、VEGF为阴性情况下侵袭性垂体腺瘤与非侵袭性组差异均无统计学意义（t=1.56、1.47、0.53、0.21,均P＞0.05）;经相关性分析,侵袭性垂体腺瘤患者MMP二9与VEGF表达水平呈正相关（r=0.734,P＜0.01）.结论 侵袭性垂体腺瘤组织中MMP-9与VEGF表达增高,MMP-9与VEGF可作为辅助垂体腺瘤侵袭性判断的生物学指标.

Objective To investigate the relationship in clinical features of invasive pituitary adenoma patients and their aggressive,matrix metalloproteinase 9 （MMP-9）,vascular endothelial growth factor （VEGF） expression and tumor invasiveness of pituitary adenomas.Methods 96 patients with pituitary adenoma were selected,46 cases of invasive pituitary adenomas,50 cases of non-invasive pituitary adenomas,MMP-9,VEGF expression were detected by immunohistochemical assay,their expression levels were analyzed with tumor invasiveness correlation.Results MMP-9,VEGF expression in invasive pituitary adenomas strongly positive rates were 54.35%,52.17%,the total positive rates were 89.13%,95.65%,which were significantly higher than the non-invasive pituitary adenomas MMP-9,VEGF strong positive expression rate of 16.00%,20.00%,the total positive rates were 44.00%,56.00% （χ2 =15.618,9.416,21.631,20.090,all P 〈 0.05）;MMP-9,VEGF for invasive pituitary volume under positive circumstances,the maximum diameter was significantly higher than non-invasive of the group （t =4.32,3.67,2.49,2.54,all P 〈 0.05）,and MMP-9,VEGF were under negative circumstances invasive and non-invasive pituitary adenoma group,the difference was not statistically significant（t =1.56,1.47,0.53,0.21,all P 〉 0.05）;By correlation analysis,invasive pituitary adenoma MMP-9 expression was positively correlated with VEGF levels （r =0.734,P 〈 0.01）.Conclusion Invasive pituitary adenomas of MMP-9 and VEGF expression increased,MMP-9 and VEGF can be used as auxiliary judge biological indicators of invasive pituitary adenomas.