一株紫色非硫光合细菌的分离鉴定和培养特性研究

Isolation,Identification and Cultural Character of a Strain of Purple Nonsulfur Photosynthetic Bacteria

[目的]为了获得功能光合细菌新菌源.[方法]从茂名市郊区池塘底泥样中分离、纯化得到一株紫色非硫光合细菌,编号为GB-1.根据菌株的形态、细胞色素光吸收特征及生理生化分析,对分离菌GB-1进行鉴定,并且研究该菌的培养特性.[结果]经初步鉴定,该菌为沼泽红假单胞菌（Rhodopseudomonas palustris）.该菌的最适生长条件为：以小分子有机酸为碳源,pH 8 ～9,32～35℃,NaCl浓度小于1％,白炽灯光照微氧.[结论]该菌能耐受浓度5％ NaC1,培养7d后NH4＋-N、NO2--N的转化率分别达到57.71％和47.45％,在水质净化方面显示较好的应用前景.

[ Objective] The research aimed to obtain a new bacteria source of functional photosynthetic bacteria. [ Method ] A strain of purple nonsulfur photosynthetic bacteria GB-1 was isolated and purified from the sediment of fishpond in Maoming suburbs. The classification and i- dentification of purple nonsulfur photosynthetic bacteria GB-1 was carried out by the observation of cell morphology, the cell coloring matter absorption spectrum determination and the study of physiological and biochemical characteristics. Meanwhile, the cultural character of the strain GB-1 was subsequently investigated. [ Resuh] The strain GB-1 was identified as Rhodopseudomonas palustris. The optimum conditions for its growth were studied as follows ： the best carbon source was a small molecule organic acid, growth temperature was 32 - 35 ℃, pH was 8 - 9, NaCl concentration was less than 1%, and the condition of illumination was incandescent light bulb and micro-aerobic. [ Conclusion ] Halotolerated ability of GB-1 were 5%. After the cultivation for 7 d, the removal rates for ammonia nitrogen and nitrite by the strain GB-1 was up to 57.71% and 47.45%, respectively. It appeared broad application prospects in the treatment of eutrophic water.