白念珠菌CaFIG1基因缺失突变株的构建

Construction of Ca FIG1 Gene Deletion Mutant in Canidia albicans

[目的]研究Ca FIG1基因除参与有性生殖过程外,是否还参与细胞钙稳态等其他功能,对Ca FIG1进行基因敲除。[方法]采用2种方法分别敲除Ca FIG1的2个等位基因,一种通过PCR扩增HIS1敲除盒,另一种通过克隆的手段构建URA3敲除盒。[结果]从白念珠菌RM1000中成功敲除了Ca FIG1双等位基因。[结论]该敲除策略同样适用于其他基因的敲除,提高了基因敲除的效率。

[Objective] The aim was to construct CaFIG1 gene deletion mutant in Canldia albicanstostudy the additional functions of CaFIG1 gene ,such as participate in the regulation of cell calcium homeostasisits besides in mating. [ Method] Two methods were adopted to knockout two alleles of CaFIG1 gene. One was HIS1 knockout cassette by PCR, the other was URA3 knockout cassetteby clone. [ Resuh ] The study successfully knockout two alleles of CaFIG1 gene in C. albicarts strains RM1000. [ Conclusion] This knockout strategy was also applicable to knockoutother gene, and it would improve the efficiency of gene knockout.