黄菠萝嫩茎离体培养再生体系的建立

Establishment for Regeneration System of Younger Stem of Phellodendron amurense Rupr. in vitro

[目的]优化黄菠萝离体培养再生体系。[方法]以黄菠萝带腋芽的嫩茎为外植体,采用正交设计研究其离体培养再生体系。[结果]诱导黄菠萝的最佳初代培养基是MS+1.5 mg/L 6-BA+0.06 mg/L NAA+20 g/L蔗糖,继代培养的最佳培养基组合是MS+1.0mg/L 6-BA+0.1 mg/L NAA,最佳生根培养基组合是1/2MS+0.4 mg/LNAA+30 g/L蔗糖。[结论]以黄菠萝带腋芽的嫩茎为外植体,采用正交设计研究其离体培养和植株再生技术方法是可行的。

[ Objective ] To optimize Phellodendron amurense Rupr. in vitro culture regeneration system. [ Method ] By using axillary bud-bearing young stem of Phellodendron amurense as explants, the orthogonal experimental design was employed to study the in vitro culture and regeneration condition. [Result] The optimum primary medium was MS ＋ 1.5 mg/L 6-BA ＋0.06 mg/L NAA ＋20 g/L sucrose, the best grouch regulatory substance combination of medium for subculture was MS ＋ 1.0 mg/L 6-BA ＋0.1 mg/L NAA, and the best rooting medium was 1/2MS ＋0.4 mg/LNAA ＋ 30 g/L sucrose. [ Conclusion] Taking axillary bud -bearing young stem of PheUodendron amurense as explants, adopting orthogonal design to study its in vitro culture and plants regeneration technique is feasible.