银杏叶提取物对急性脑梗死大鼠凋亡基因的影响

Effects of Ginkgo biloba extract on apoptosis related gene expression of rats with acute cerebral infarction

目的:探讨了银杏叶提取物对急性脑梗死大鼠凋亡基因的影响。方法:运用线栓法构建66只大鼠急性脑梗死模型,并随机分为假手术组、模型组和治疗组,每组22只,其中假手术组只分离血管不插线。治疗组大鼠给予尾静脉注射银杏叶提取物2.5 mg·kg-1,假手术组和模型组均给予尾静脉注射等体积的PBS溶液。治疗72 h后处死大鼠,取脑组织,采用实时荧光定量PCR分析3组大鼠脑组织中Bcl-2、Bax和caspase-3 m RNA水平;采用免疫组化法测定脑组织凋亡基因相关蛋白Bcl-2、Bax和caspase-3的表达;采用TUNEL染色法分析脑细胞凋亡指数。并分析10 d内3组大鼠的生存曲线。结果:与假手术组比较,建模后模型组和治疗组大鼠脑组织中Bcl-2 m RNA Bax和caspase-3 m RNA和蛋白质水平显著升高(P<0.05)。经治疗后,治疗组大鼠脑组织中caspase-3和Bax m RNA和蛋白质水平较模型组明显下降(P<0.05),而Bcl-2 m RNA和蛋白质水平较模型组显著升高(P<0.05)。建模后,模型组和治疗组大鼠脑组织细胞凋亡指数较假手术组显著升高加(P<0.05),治疗组大鼠治疗后脑组织中细胞凋亡指数较模型组显著下调(P<0.05)。与假手术组比较,模型组和治疗组大鼠10 d内存活率明显下降,但治疗组存活率明显高于模型组(P<0.05)。结论:银杏叶提取物能够有效调节急性脑梗死大鼠凋亡基因及其相关蛋白的表达,从而降低脑组织细胞的凋亡,对脑组织具有一定的保护作用。

OBJECTIVE To investigate the effects of Ginkgo biloba extract on apoptosis related gene expression of rats with acute cerebral infarction.METHODS Rat models with acute cerebral infarction were constructed by suture method, and were randomly divided into sham group （only isolated vascular suture unplugged, n = 22）, model group （n = 22） and treatment group （n = 22）. Rats in treatment group were given Ginkgo biloba extract at dose of 2. 5 mg·kg^-1 and rats in sham operation group and model group were administered PBS solution by intraperitoneal injection. After 72 h, the rats were sacrificed and brain tissues were separated. Bcl-2, Bax and easpase-3 mRNA and protein levels were determined by real time quantitative PCR and immunohistochemistry. Apoptosis index of brain tissues was analyzed by TUNEL staining. And the survival curve of rats was analyzed. RESULTS Compared with sham group, Bcl-2, Bax and caspase-3 mRNA levels in model group and treatment group increased significantly （P〈0. 05）. After treatment, Bax and caspase-3 mRNA levels of brain tissues of rats in treatment group decreased significantly than that of model group （P〈0. 05）, and Bcl-2 mRNA level increased significantly than that of model group （P〈0. 05）. Apoptotic levels of brain tissues in model group and treatment group significantly increased compared with sham group （P〈0. 05）, while apoptotie levels of brain tissues in treatment group significantly decreased than that of mod- el group （P〈0. 05）. Compared with sham group, survival rates in model group and treatment group significantly reduced （P〈 0. 05）, while survival rate in treatment group was higher than that of model group （P〈0. 05）. CONCLUSION Ginkgo biloba extract can effectively regulate apoptosis of rats with acute cerebral infarction and its related gene proteins. It can reduce apoptosis of brain cells and has protective effects on brain tissues.