新型生物转笼工艺处理榨菜废水的微生物群落研究

Study on Microbial Communities in New Packed Cage Rotating Biological Contactor for Mustard Wastewater Treatment

研发了一种新型生物转笼工艺,在鱼泉榨菜废水处理站进行启动,并应用光学显微镜镜检和PCR-DGGE技术就启动期间反应器中的微生物群落结构进行分析。结果表明,该工艺比传统转盘工艺具有更强的有机物去除能力,逐步提升负荷的启动方式对功能菌的发育更为有利。镜检显示,转盘前端长期处于丝状菌膨胀状态,而转笼生物膜则较为密实,表现出更强的适应性。DGGE图谱分析表明,反应器对接种混合菌种进行了"智能化"筛选,生物膜样品与接种污泥的相似性最高仅为57.7%,但各反应器末端生物膜与接种污泥的相似性均高于前端,丰富度和Shannon指数值呈现同样趋势。

A new packed cage rotating biological contactor was developed and started up in Yu- quan mustard wastewater treatment station. The microbial communities in the reactor during the start-up phase were analyzed by using optical microscopy and PCR-DGGE. The results indicated that the packed cage rotating biological contactor had higher capacity for removing organics than the traditional rotating bi- ological contaetor, and increasing the load gradually was beneficial for the development of functional bac- teria. Microscopic examination revealed that filamentous bulking always existed in the front of the tradi- tional rotating biological contactor, while the packed cage rotating biological contactor was more adaptable to the circumstance with dense biofilm. Meanwhile, DGGE profiles indicated that the intelligent screening of the seeding sludge was performed in the reactor. The highest similarity between the samples and the seeding sludge was only 57.7%, but the similarity between the samples and the seeding sludge at the endof each reactor was higher than that in the front. Richness and Shannon index showed the same trend.