复方苦参注射液对人肝癌SMMC7721细胞增殖的抑制作用

Inhibitory Effects of Compound Kushen Injection on the Proliferation of Human Hepatoma SMMC7721 Cells

目的:研究复方苦参注射液对人肝癌SMMC7721细胞增殖的抑制作用。方法:体外传代培养SMMC7721细胞。10、20、40、80、160μl/ml复方苦参注射液培养细胞24、48、72 h后,采用MTT法测定细胞活力并计算抑制率。0(阴性对照)、10、20、40、80、160μl/ml复方苦参注射液培养细胞48 h后采用流式细胞仪测定细胞凋亡率,并分析细胞周期分布百分比。结果:10、20、40、80、160μl/ml复方苦参注射液培养细胞24、48、72 h对细胞具有明显的抑制作用,且具有时间和剂量依赖性。与阴性对照比较,40、80、160μl/ml复方苦参注射液培养细胞48 h后,G0/G1期细胞比例明显升高(P<0.05),S期细胞比例明显降低(P<0.05),细胞凋亡率明显升高(P<0.05)。结论:复方苦参注射液对SMMC7721细胞的增殖具有抑制作用,其机制可能是诱导细胞凋亡、阻滞细胞于G0/G1期。

OBJECTIVE： To study the inhibitory effects of Compound kushen injection on the proliferation of human hepatoma SMMC7721 cells. METHODS： Human hepatoma cell line SMMC7721 was subcultured in vitro. MTT assay was adopted to deter- mine cell viability after the cells were cultured in 10μl/ml, 20μl/ml, 40μl/ml, 80μl/ml and 160μl/ml Compound kushen injec- tion for 24 h, 48 h and 72 h, and the inhibition rates were calculated. The apoptosis rates was determined after the cells were cul- tured in 0 （negative control）, 10 μl/ml, 20μl/ml, 40μl/ml, 80 μl/ml and 160μl/ml Compound kushen injection for 48 h,and the cells were cultured therein for 48 h, and percentage of cell cycle distribution was analyzed. RESULTS： After the cells were cul- tured in 10μl/ml, 20μl/ml, 40μl/ml, 80μl/ml and 160μl/ml Compound kushen injection for 24 h, 48 h and 72 h, the injection showed obvious inhibitory effect on the cells with time and dose-dependence. Compared with negative control group, the proportion of the cells in G0/G1 phase was obviously increased （P〈0.05） and that of the cells in S phase was obviously decreased （P〈0.05）, and the apoptosis rates of the cells were obviously increased（P〈0.05） after the cells were cultured in 40μl/ml, 80 μl/ml and 160 μl/ml Compound kushen injection for 48 h. CONCLUSIONS： Compound kushen injection have inhibitory effects on the prolifera- tion of SMMC7721 cells by a mechanism that may be related to the induction of apoptosis and G0/G1 cell cycle arrest.