血栓心脉宁片对血管性痴呆模型大鼠的保护作用

Protection Effects of Xueshuan Xinmaining Tablet on Model Rats with Vascular Dementia

目的:研究血栓心脉宁片对血管性痴呆(VD)模型大鼠的保护作用。方法:通过双侧颈总动脉反复夹闭再灌注同时ip给予硝普钠(2.5 mg/kg)以复制大鼠VD模型。实验分为正常对照[等容0.5%羧甲基纤维素钠(CMC-Na)溶液]组、模型(等容0.5%CMC-Na溶液)组、吡拉西坦(0.5 g/kg)组与血栓心脉宁高、低剂量(2.2、1.1 g/kg)组,ig给药,每天1次,连续2个月。水迷宫法测定学习期(前6 d)大鼠潜伏期、游程,测试期(第7天)大鼠穿台次数、穿过有效区次数、平台停留距离、平台停留时间、有效区停留距离、有效区停留时间及相应比值。制作大鼠脑部切片并通过显微镜进行大鼠海马与皮质病理形态学观察。结果:在学习期,与正常对照组比较,模型组第1、2、3、4、6天大鼠潜伏期延长、游程增加(P<0.01或P<0.05);与模型组比较,血栓心脉宁高、低剂量组每时间点潜伏期与游程均有一定缩短(P<0.01或P<0.05)。在测试期,与正常对照组比较,模型组除有效区停留距离、有效区停留距离/总路程之比、有效区停留时间/总时间之比以外,其余指标均减少(P<0.01或P<0.05);模型组大鼠皮质、海马神经细胞数减少,噬神经现象较多。与模型组比较,血栓心脉宁高、低剂量组各指标均有一定改善(P<0.01或P<0.05)。结论:血栓心脉宁对VD大鼠学习记忆能力具有改善作用,能提高其定位航行和空间探索能力,改善受损海马及皮质组织。

OBJECTIVE： To study the protective effects of Xueshuan xinmaining tablet on model rats with vascular dementia （VD）. METHODS： Bilateral carotid artery repeating occlusion reperfusion and ip sodium nitroprusside （2.5 mg/kg） were used to reproduce VD rat models. There were normal control group [isovolumetric 0.5% sodium carboxymethyl cellulose （CMC-Na） solu- tion], model group （isovolumetric 0.5% CMC-Na solution）, piracetam group （0.5 g/kg） and Xueshuan xinmaining high and low dose groups （2.2 and 1.1 g/kg）, ig, once a day, for 2 months. Water maze was used to test the incubation period, run length of rats in the learning period （the first 6 days） and the times of stage crossing and effective areas crossing, stop distance and stop time of stage, stop distance and stop time of effective areas and the ratios of rats in test period （the 7th day）. Brain slices of rats were made and inverted microscope was used for brain pathology observation. RESULTS： During the learning period, compared with normal control group, the incubation period in model group was prolonged in 1, 2, 3, 4 and 6 d and run length was in- creased （P〈0.01 or P〈0.05） ; compared with model group, the incubation period and run length in Xueshuang xinmailming high and low dose groups were shortened at each time point （P〈0.01 or P〈0.05）. During test period, compared with normal control group, the other indexs were desreased in model group except for step distance of effective areas, stop distance to total distance and stop time to total time （P〈0.01 or P〈0.05）. The cortex and hippocampus tissue of rats in model group were decreased in nerve cells and there was more neurological phagocytosis. Compared with normal control group, all indexes were improved in Xue- shuang xinmainning high and low dose groups （P〈0.01 or P〈0.05）. CONCLUSIONS： Xueshuan xinmaining tablet can improve the learning and memory of rats with VD, positioning navigation, space exploration capabilities and the damaged hippocampus and cortex.