电针足三里对对乙酰氨基酚诱发大鼠肝损伤炎症细胞因子的影响

Effect of Acupuncture at Zusanli Acupoint on Inflammatory Cytokines of Hepatic Injury With Given Multiple Doses of Paracetamol in Rats

目的:研究电针足三里对对乙酰氨基酚(Acetaminophen,APAP)诱发大鼠肝损伤炎症细胞因子水平的影响,探讨其对药源性肝损伤的保护作用。方法:SD大鼠按随机数字表法分为NC组、APAP1组和Acup1组、APAP2组和Acup2组,各组大鼠连续给药或针刺14 d后,检测大鼠血清和肝脏组织匀浆液白细胞介素6(IL-6)、白细胞介素8(IL-8)和肿瘤坏死因子α(TNF-α)的含量。结果:与APAP1组和APAP2组比较,Acup1组和Acup2组大鼠血清谷草转氨酶(AST)和谷丙转氨酶(ALT)活性水平均显著性升高。与APAP1组和APAP2组比较,Acup1组和Acup2组大鼠血清和肝脏匀浆组织液中IL-6、IL-8和TNF-α含量水平均显著升高。结论:电针足三里上调APAP诱导大鼠肝损伤血清和肝脏组织炎症细胞因子的反应和表达水平,可能会对APAP诱导的肝脏损伤具有一定的促进作用。

Objective ： To investigate the effects of acupuncture at Zusanli aeupoint on inflammatory eytokines changes in rats with given multiple doses of paracetamol, and the protective effect of acupuncture on hepatic injury. Methods： SD rats were divided into five groups, the NC group, the APAP1group and the Acupl group,the APAP2 group and the Acup2 group. Each group was given paracetamol or saline once a day for 14 continuous days, and the serum levels of alanine transaminase（ALT） and aspartate transaminase （AST）of the rats were measured, and the levels of Interleukin 6 （IL-6）, Interleukin 8 （IL-8）, tumor necrosis factor-α （TNF-α） in serum and liver tissue homogenate were determined by euzymelinked immunosorbent assay（ELISA） respectively. Results： Compared with groups of APAPl andAPAP2, the levels of serum AST and ALT were significantly increased in groups of Acup1 and Acup2. And compared with groups of APAP~ andAPAP2, the levels of IL-6,IL-8 and TNF-α in the serum and liver were significantly increased in groups of Acup1 and Acup2. Conclusion ： The results suggest that electroacupuncturing Zusanli acupoints can increase levels of inflamatory IL-6, IL-8 and TNF-α in the serum and liver, and may promote liver damage induced by paracetamol.