Caspase 3-like蛋白酶和TaeMCAⅡ基因对小麦胚乳细胞PCD的影响

Effects of Caspase 3-like proteases and TaeMCAⅡon the programmed cell death of wheat endosperm under waterlogging

对正常生长和淹水处理的华麦8号胚乳细胞发育过程中Caspase 3-like蛋白酶活性和TaeMCAⅡ基因表达量进行了研究。结果显示,正常生长条件下,在胚乳细胞发生PCD早期(花后12d),Caspase 3-like蛋白酶活性较强,且该酶主要定位于胚乳细胞中的淀粉质体上;整个胚乳细胞PCD过程中TaeMCAⅡ基因表达量变化不大。在淹水胁迫处理下,胚乳TaeMCAⅡ基因表达量和Caspase 3-like蛋白酶活性比同期正常处理高。上述研究结果表明,Caspase 3-like蛋白酶参与了正常生长的胚乳细胞PCD过程;淹水胁迫会导致TaeMCAⅡ基因超表达和Caspase 3-like蛋白酶活性升高,这可能是胚乳细胞PCD进程提前的原因之一。

Agricultural University;College of Plant Sciences and Technology,Huazhong Agricultural University; The Caspase 3-like protease activity and TaeMCAⅡ gene expression during the process of programmed cell death（PCD）in wheat（Hua 8）endosperm under normal and waterlogging treatments were studied in this paper.The results indicated that,under normal treatments,the activity of Caspase 3-like protease was higher in the previous phase of endosperm PCD（12DAF）,and the protease was mainly located in amyloplasts of wheat endosperm cells,while the expression of TaeMCAⅡ gene was similar during endosperm PCD.The Caspase 3-like protease activity and the expression of TaeMCAⅡ gene were higher in wheat endosperm after waterlogging than control groups in same days after flowering.Above all,the Caspase 3-like protease participates in the PCD progression of control wheat endosperm.The rise of the overexpression of TaeMCAⅡ gene and Caspase 3-like activity may cause the endosperm PCD happened earlier under waterlogging.