3种化学预处理下水葫芦和甘蔗渣的酶解产糖

Sugar production of water hyacinth and sugarcane bagasse biomass by enzymatic hydrolysis under three kinds of chemical pretreatment

采用2%(m/V)H2SO4(稀酸)、2%NaOH(稀碱)和1%H2O2+2%NaOH(氧化+稀碱)3种化学方法对水葫芦秸秆和甘蔗渣进行预处理后酶解,分析预处理后各样品主要化学组分含量变化及其去除率与酶解效果之间的关系。结果表明,对水葫芦来说,3种预处理后的酶解产糖率差异不大,而对甘蔗渣来说,稀碱和氧化+稀碱预处理下的酶解产糖率显著高于稀酸预处理下的产糖率,并高于3种预处理后水葫芦样品的酶解产糖率,显示出甘蔗渣作为产糖原料的优越性。经预处理后的样品酶解产糖率与纤维素含量及木质素和灰分的去除率成正比。氧化+稀碱预处理后甘蔗渣样品在12h的还原糖产率达81.54%,该预处理温度较低,原料利用率较高,是一种有效的预处理方法。

Three kinds of chemical pretreatment including 2% H2SO4,2% NaOH,1% H2O2＋2%NaOH were used to pretreat water hyacinth and sugarcane bagasse biomasses.The pretreated biomasses were subjected to enzymatic hydrolysis.The relationship between the variation and the removal of the main components in pretreated biomasses and the effects of sugar production by enzymatic hydrolysis were investigated.Results showed that the yields of reducing sugar of water hyacinth pretreated by three chemical pretreatments had no obvious differences.The yields of sugarcane bagasse pretreated with2% NaOH and 1% H2O2＋2% NaOH were significantly higher than that pretreated with 2% H2SO4,and higher than those of water hyacinth under three kinds of pretreatment,indicating that it was superior to water hyacinth.Cellulose content,lignin and ash removal had a positive effect on yield of reducing sugar of samples pretreated.High yield of reducing sugar up to 81.54% was obtained by enzymatic hydrolysis of sugarcane bagasse pretreated with 1% H2O2 ＋2% NaOH for 12 hours.1% H2O2 ＋2%NaOH is an effective pretreatment method for lignocellulose materials due to its relatively mildness and high efficiency of utilizing raw material.