响应面法优化胆黄连的炮制工艺

Optimization of Processing Technology of Bile Processed Rhizoma Coptidis Using Response Surface Methodology

目的:优选炒制胆黄连的炮制工艺参数。方法:以总生物碱、浸出物含量的归一值OD为因变量,运用Box-Behnken试验考察炒药温度、炒药时间、闷润时间对胆黄连炮制工艺的影响,运用Design Expert 8.0.6软件对试验数据进行分析,建立OD值与各自变量的多元二次回归方程的数学模型,通过响应面法确定工艺参数。采用紫外分光光度法测定总生物碱的含量。结果:胆黄连的最佳炮制工艺是炒药温度为94℃,炒药时间为19min,闷润时间为1 h。此模型下胆黄连中总生物碱含量为131.494 mg·g-1,浸出物含量为5.243%。回归模型的预测值与实测值的相对误差<3%,该回归方程与实际情况拟合较好。结论:采用响应面法优化的胆黄连炮制工艺可用于胆黄连饮片制备。

Objective： To optimize the best processing technology of bile processed Rhizoma Coptidis,response surface methodology was used. Methods： According to OD value of the contents of total alkaloids and extract of bile processed Rhizoma Coptidis for index,Box- Behnken experimental design was adopted to investigate the effects of the three main factors including temperature,time of fried medicine,time of covered moistening. Experimental data analysis was disposed by Design Expert 8. 0. 6 software,multivariate quadratic regression equation mathematical model between composite score and independent variables was established,and then technical parameters were determined by response surface methodology.Ultraviolet spectrophotometry（ UV） was employed to determine the contents of total alkaloids in the bile processed Rhizoma Coptidis. Results： Based on the results of response surface methodology,the best processing technology parameter was ascertained as follows： temperature was 94 ℃,time of freid medicine was 19 min,time of covered moistening was 1 h. Yield of total alkaloids of that optimized processing technology was 131. 494 mg·g- 1. The determination of extract of bile processed Rhizoma Coptidis was 5. 243%. The experimental values agreed with those predicted within a 3%relative error,indicating the suitability of response surface methodology in optimizing processing technology of bile processed Rhizoma Coptidis. Conclusion： The response surface method can be used in the research of traditional Chinese medicine（ TCM） processing technology.