马钱子配伍白术前后主要生物碱成分变化的HPLC测定

Determination of Brucin and Strychnine in Strychnos Nux-vomical Uncombined and Combined with Atractylodes Macrocephala Koidz by HPLC

目的:通过马钱子配伍白术(马钱子配伍白术以下简称马白)不同比例水煎液中马钱子碱和士的宁的变化,观察配伍对马钱子主要毒性成分的影响,并寻求减毒较佳配伍比例。方法:采用HPLC分别对配伍前后主要生物碱成分马钱子碱和士的宁的含量进行测定。结果:与马钱子单煎剂比较,马白1∶2煎剂中马钱子碱和士的宁分别升高17.6%和46.9%;马白1∶4煎剂的马钱子碱和士的宁别下降23.5%和8.5%;马白1∶6煎剂中马钱子碱和士的宁分别降低29.0%和16.6%。结论:马白1∶4、1∶6煎剂可降低马钱子毒性成分,起到减毒作用。减毒较佳配比为马白1∶6。

Objective： Through the combination of Strychnos Nux-vomical and Atractylodes macrocephala Koidz（ hereinafter referred to as SA）,the article observes the impact on Strychnine and Brucine of different proportions,and tries to seek the better proportion. Method： Use HPLC to determinate the mass fraction of strychnine and brucine,which in Strychnos Nux-vomical uncombines and combines with Atractylodes macrocephala Koidz. Results： Comparing with Strychnos single decoction,SA 1 ∶ 2 decoction＇s brucine and strychnine has increased 17. 6% and 46. 9%; SA 1 ∶ 4 decoction＇s brucine and strychnine has decreased 23. 5% and 8. 5%; SA 1 ∶ 6 decoction＇s brucine and strychnine has decreased 29. 0% and16. 6%. Conclusion： SA 1 ∶ 4,1 ∶ 6 decoction can reduce the toxic components of Strychnos Nux- vomical. The better proportion which can reduce the toxic components is SR 1 ∶ 6.