华南地区猪流行性腹泻病毒的分子流行病学调查与分析

Investigation and analysis on molecular epidemiology of porcine epidemic diarrhea virus in south China

为了解华南地区猪流行性腹泻病毒(PEDV)的流行、变异及进化情况,从该地区的不同猪场采集2 159份样品,利用RT-PCR方法检测PEDV,扩增了部分PEDV阳性样品中的S、M和ORF3基因并测序,利用生物信息学软件进行序列分析。结果显示,PEDV在猪场中的检出率为100%,在所有样品中的平均检出率为53.0%,在哺乳仔猪、断奶仔猪、育肥猪和母猪样品中,PEDV平均阳性率分别为67.6%、42.8%、12.8%和47.2%。根据S、ORF3和M基因建立的进化树,PEDV可分为2个基因群,其中当前样品株为一群,韩国、美国和中国的参考毒株为一群。结果表明,PEDV在华南地区猪场普遍存在,其基因也在不断发生变化。

In order to know the epidemic, variation and evolution of porcine epidemic diarrhea virus （PEDV） in south China, 2159 samples were collected from different herds in south China. In this study, partial spike （S）, ORF3, and membrane （M） genes amplified from these samples were sequenced and analyzed. While, the morbidity and mortality rate of herds mentioned above was counted. The results showed that all of the herds were positive for PEDV, the average positive rate of PEDV was 53.0%. However, the mean positive rate of PEDV in suckling piglets, nursing piglets, finishing pigs and sows were 67.6% ,42.8%, 12.8% and 47.2%, respectively. The phylogenetie trees that were constructed by MEGA based on partial S, M and ORF3 genes, showed that all the PEDV analyzed fell into two groups, which were consisted by present fields strains and reference strains. These results indicated that PEDV was prevalent in the field herds in south China and variant in PEDV genes.