福州日溪野生蕉自然群体遗传结构的ISSR分析

ISSR analysis on genetic structure of natural population in wild banana from Rixi town, Fuzhou city

以福州市日溪乡自然居群的38份野生蕉样品为材料,采用ISSR分子标记并结合相关软件进行遗传结构分析。结果表明:筛选的13条引物共扩增得到116条DNA条带,其中多态性条带为70条、占60.34%,说明福州日溪野生蕉自然群体具有较高水平的遗传多样性;通过NTSYS和STRUCTURE聚类分析将38份野生蕉样品划分为6大类,结合Q值分析表明,福州日溪野生蕉群体内部存在着一定的遗传信息交流。

Thirty-eight samples of wild banana leaves from Rixi town of Fuzhou were collected as materials, the technical system of ISSR marker was used, and their genetic structures were analyzed by related software STRUCTURE and NTSYS in the study. The results showed that based on 13 primers, 116 stable DNA fragments were obtaimed, of which polymorphic band number was 70, accounting for 60.34%,which revealed a relative high level of genetic diversity. The results of NTSYS and STRUCTURE analysis showed that 38 wild materials could be divided into 6 groups. The Q-value analysis showed that there was a certain exchang of genetic information in the natural populations of wild banana in Rixi town, Fuzhou city.