佛山市南海区性病门诊350例疑似生殖器疱疹患者单纯疱疹病毒抗原与抗体检测与分析

Detection and analysis of herpes simplex virus antigens and antibodies in patients with suspected genital herpes among STD clinic attendants in Nanhai District Foshan

目的了解佛山市南海区性传播疾病(STD)门诊病人单纯疱疹病毒感染的流行情况。方法对2013年1月—2014年6月间前往佛山市南海区3个规范化性病门诊就诊的患者进行问卷调查、生殖器疱疹临床诊断和单纯疱疹病毒分类抗体检测、分泌物实时荧光定量聚合酶链反应(FQ-PCR)检测。结果 350例疑似生殖器疱疹患者中,HSV-I的Ig M和Ig G的检出率分别是5.4%(19/350)和87.7%(307/350),HSV-II的Ig M和Ig G检出率分别是2.9%(10/350)和42%(147/350),两种血清型中以HSV-I感染为主,且Ig G阳性率高于Ig M(HSV-I的χ2=238.1,P<0.005;HSV-II的χ2=259.08,P<0.005);HSV-ⅡIg G感染者147例(男115例,女32例),感染率分别为41.2%和42.7%。男性HSV-ⅡDNA感染率高于女性,二者差异有统计学意义(χ2=3.94,P<0.05)。结论本地区性病门诊中HSV抗体检出率高,以HSV-I感染为主;对于疑似生殖器疱疹患者,同时进行HSV抗原及血清HSV-I、HSV-II的Ig M和Ig G抗体联合检测更有助于临床GH诊断;男性患者易于临床诊断。

Objective To investigate the epidemiological characteristics of herpes simplex virus infection in patients at- tending a sexually transmitted diseases （STD） clinic in Nanhai District of Foshan. Methods The 350 patients attending the three standardized STD clinic in Nanhai District of Foshan from January ,2013 to June ,2014 were interviewed with an anonymous questionnaire, blood-tested for HSV（ IgM ,IgG） antibody. And secretion was detected by FQ-PCR for HSV antigen . Results In 350 patients with suspected GH,the positive rate of HSV-Ⅰ IgM and IgG were 5.4% （19/350）and 87.7% （307/350）. The positive rate of HSV-Ⅱ IgM and IgG were 2. 9% （10/350） and 42% （147/350） ,respectively. HSV-Ⅰ infection was principal in two serologic types, and the positive rate of IgG was higher than that of IgM （ P 〈 0. 05 ）. There were 147 patients infected HSV-Ⅱ IgG （male 115 ,female 32）. The infection rate is 41.2% and 42. 7%. The infection rate of HSV- Ⅱ DNA in men was higher than that in women. There was a significant difference between them（ P 〈 0.05 ）. Conclusion There was a high positive detection rate of HSV from sexually transmitted disease clinic in this region. And HSV-1 was principal. For patients with suspected genital her- pes ,detection of HSV antigen tests combined with HSV-Ⅰ,HSV-Ⅱ IgM and IgG serum antibody tests is more helpful in the clinical diagnosis of genital herpe. HSV infected men patients were likely to clinically diagnose.