爬山虎水提液与抗菌药联用对含fosA3基因大肠杆菌的抑菌效果

Antibacterial effect of Parthenocissus tricuspidata water extract combined with antimicrobial on Escherichia coli containing fosA3 gene

【目的】探讨爬山虎水提液与抗菌药联用对含fos A3基因大肠杆菌的抑菌效果,为爬山虎的综合开发利用提供参考依据。【方法】从病猪的肠道组织分泌物中分离菌株,对其进行鉴定和fos A3基因检测,采用二倍微量稀释法检测爬山虎水提液与不同抗菌药的最小抑菌浓度（MIC）,然后用微量棋盘稀释法测定爬山虎水提液与抗菌药联用的抑菌浓度指数（FICI）。【结果】分离获得的菌株为含fos A3基因大肠杆菌,爬山虎水提液对含fos A3大肠杆菌的MIC为500g/m L。以500 g/m L爬山虎水提液与头孢曲松钠、磷霉素、粘杆菌素、痢菌净、磺胺间甲氧嘧啶、阿米卡星、头孢噻呋钠、氟苯尼考、头孢噻肟等9种抗菌药联用的FICI为0.188~1.000,其中,爬山虎水提液与粘杆菌素、氟苯尼考间存在协同作用,与头孢曲松钠、磷霉素、痢菌净、磺胺间甲氧嘧啶、阿米卡星、头孢噻呋钠、头孢噻肟呈相加作用。【结论】爬山虎水提液可增强部分抗菌药对含fos A3大肠杆菌的抗菌活性,且呈协同或相加作用。

[Objective]The antibacterial effect of Parthenocissus tricuspidata water extract combined with antimicrobial on Escherichia eoli containing rosA3 gene was studied to provide references for comprehensive development and utilization of P. tricuspidata. [ Method ]The bacterial strain isolated from diseased intestinal tract was identified and its rosA3 gene was detected. Then the minimal inhibitory concentration （MIC） and fractional inhibitory concentration indexes （FICI） of P. tricuspidata water extract combined with antimierobials were determined by using twice microdilution and mierodilution checkerboard methods, successively. [Result]The results showed that, the isolated acterial strain was found out to be E. coli containing rosA3 gene, and the MIC of P. tricuspidata water extract against it was 500 g/mL. When P. tricuspidata water extract of 500 g/mL were combined with 9 antimicrobials viz., Ceftriaxone Sodium, Fosfomycin, Coly-mycin, Mequindox, Sulfamonomcthoxine, Amikacin, Ceftiofur sodium, Florfenicol, Cefotaxime sodium, respectively, the FICI ranged from 0.188 to 1.000. There was synergistic effect between P. tricuspidata water extract and Coly-mycin or Florfenicol, meanwhile there was additive effect between it and any of other 7 antimicrobials viz., Ceftriaxone sodium, Fosfomycin, Mequindox, Sulfamonomethoxine, Amikacin, Ceftiofur sodium and Cefotaxime sodium, respectively. [Conclusion ]The antibacterial activity of some antibacterials against E. coli containing rosA3 gene were enforced by P. tricuspidata water extract, which shows synergistic effect or additive effect.